TY - GEN
T1 - Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment
AU - Xu, Kailei
AU - Ganapathy, Kavya
AU - Wang, Zi
AU - Copland, John A.
AU - Chakrabarti, Ratna
AU - Florczyk, Stephen J.
N1 - Funding Information:
References:[1] D.W. Hutmacher et al, Cell Press 28(3) (2010) 125-33. [2] B.M. Baker et al, J Cell Sci 125(Pt 13) (2012) 3015-24. [3] R.A.A. Muzzarelli et al, Carbohydrate Polymers 83 (2011) 1433-1445. [4] D. Carulli, et al, Curr Opin Neurobiol 15 (2005) 116-20. [5] C. Ricciardelli, et al, Prostate 69 (2009) 761-9. [6] R. Chakrabarti, et al, Atlas of Genetics and Cytogenetics in Oncology and Haematology (7) (2011). Acknowledgement: This work was supported by UCF start-up funding (SJF).
Publisher Copyright:
© 2019 Omnipress - All rights reserved.
PY - 2019
Y1 - 2019
N2 - Statement of Purpose: Researchers have shown that 3D cultures better mimic the in vivo tumor microenvironment than 2D cultures [1]. Therefore, 3D culture of cancer cells using 3D scaffolds has been investigated to explore the interaction between cells and stroma. Composition of the 3D substrate influences cell function, signaling, and cell morphology [2]. Chitosan (C) is a commonly used biomaterial that has beneficial biological properties including hydrophilic surface, biodegradability, and biocompatibility [3]. Due to its cationic nature, chitosan can form an ionic complex by mixing with anionic polymers. Chondroitin sulfate (CS) is one of glycosaminoglycans in proteoglycans [4]. CS was found in greater amounts in advanced and metastatic prostate cancer than early stage [5]. In this study, novel 3D porous C-CS scaffolds were prepared by mixing chitosan with CS and three scaffold compositions were prepared with different CS concentrations: 0.1 wt% (4-0.1), 0.5 wt% (4-0.5) and 1 wt% (4-1) CS. The effect of CS composition in scaffolds on prostate cancer (PCa) cells was investigated by in vitro analysis with PC-3 and 22Rv1 cell lines. Our hypothesis is that PCa cultures will show higher expression of cytokeratin 8 (KRT8), androgen receptor (AR), prostate specific antigen (PSA), and LIM domain kinase 1 (LIMK1) in scaffolds with higher CS concentration.
AB - Statement of Purpose: Researchers have shown that 3D cultures better mimic the in vivo tumor microenvironment than 2D cultures [1]. Therefore, 3D culture of cancer cells using 3D scaffolds has been investigated to explore the interaction between cells and stroma. Composition of the 3D substrate influences cell function, signaling, and cell morphology [2]. Chitosan (C) is a commonly used biomaterial that has beneficial biological properties including hydrophilic surface, biodegradability, and biocompatibility [3]. Due to its cationic nature, chitosan can form an ionic complex by mixing with anionic polymers. Chondroitin sulfate (CS) is one of glycosaminoglycans in proteoglycans [4]. CS was found in greater amounts in advanced and metastatic prostate cancer than early stage [5]. In this study, novel 3D porous C-CS scaffolds were prepared by mixing chitosan with CS and three scaffold compositions were prepared with different CS concentrations: 0.1 wt% (4-0.1), 0.5 wt% (4-0.5) and 1 wt% (4-1) CS. The effect of CS composition in scaffolds on prostate cancer (PCa) cells was investigated by in vitro analysis with PC-3 and 22Rv1 cell lines. Our hypothesis is that PCa cultures will show higher expression of cytokeratin 8 (KRT8), androgen receptor (AR), prostate specific antigen (PSA), and LIM domain kinase 1 (LIMK1) in scaffolds with higher CS concentration.
UR - http://www.scopus.com/inward/record.url?scp=85065395318&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065395318&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85065395318
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
SP - 643
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -