Poly(-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells

Spencer W. Crowder; Yi Liang; Rutwik Rath; Andrew M. Park; Simon Maltais; Peter N. Pintauro; William Hofmeister; Chee C. Lim; Xintong Wang; Hak Joon Sung

doi:10.2217/nnm.12.204

Poly(-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells

Spencer W. Crowder, Yi Liang, Rutwik Rath, Andrew M. Park, Simon Maltais, Peter N. Pintauro, William Hofmeister, Chee C. Lim, Xintong Wang, Hak Joon Sung

Cardiovascular Medicine

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Aim: To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs). Materials & methods: Electrospun scaffolds of poly-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs. Results: Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(-caprolactone) scaffolds without carbon nanotube and azacytidine. Conclusion: This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium.

Original language	English (US)
Pages (from-to)	1763-1776
Number of pages	14
Journal	Nanomedicine
Volume	8
Issue number	11
DOIs	https://doi.org/10.2217/nnm.12.204
State	Published - Nov 2013

Keywords

carbon nanotube
cardiac differentiation
electrical conductivity
mesenchymal stem cell
poly-caprolactone)

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
Materials Science(all)

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10.2217/nnm.12.204

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abstract = "Aim: To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs). Materials & methods: Electrospun scaffolds of poly-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs. Results: Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(-caprolactone) scaffolds without carbon nanotube and azacytidine. Conclusion: This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium.",

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author = "Crowder, {Spencer W.} and Yi Liang and Rutwik Rath and Park, {Andrew M.} and Simon Maltais and Pintauro, {Peter N.} and William Hofmeister and Lim, {Chee C.} and Xintong Wang and Sung, {Hak Joon}",

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T1 - Poly(-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells

AU - Crowder, Spencer W.

AU - Liang, Yi

AU - Rath, Rutwik

AU - Park, Andrew M.

AU - Maltais, Simon

AU - Pintauro, Peter N.

AU - Hofmeister, William

AU - Lim, Chee C.

AU - Wang, Xintong

AU - Sung, Hak Joon

PY - 2013/11

Y1 - 2013/11

N2 - Aim: To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs). Materials & methods: Electrospun scaffolds of poly-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs. Results: Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(-caprolactone) scaffolds without carbon nanotube and azacytidine. Conclusion: This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium.

AB - Aim: To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs). Materials & methods: Electrospun scaffolds of poly-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs. Results: Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(-caprolactone) scaffolds without carbon nanotube and azacytidine. Conclusion: This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium.

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KW - cardiac differentiation

KW - electrical conductivity

KW - mesenchymal stem cell

KW - poly-caprolactone)

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Poly(-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells

Abstract

Keywords

ASJC Scopus subject areas

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