Polarized fluorescent emission from probes near dielectric interfaces

Thomas P. Burghardt

doi:10.1016/0009-3084(89)90054-6

Polarized fluorescent emission from probes near dielectric interfaces

Thomas P. Burghardt

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The electromagnetic field surrounding and emitted by a dipolar molecular probe very near to a dielectric interface is the sum of the real dipole field and the field of the image dipole induced inside the dielectric interface. The total charge distribution, made up of the real and image dipoles in close proximity to each other, approximates a quadrupole distribution and emits a light intensity pattern similar to that of an oscillating electric quadrupole. The electromagnetic field emitted by this system contains information that can be directly related to the spatial and orientational distributions of the dipole near the interface. Experimental methods are discussed that utilize this system for determining the spatial and orientational distribution of fluorescent probes in biological material.

Original language	English (US)
Pages (from-to)	271-287
Number of pages	17
Journal	Chemistry and Physics of Lipids
Volume	50
Issue number	3-4
DOIs	https://doi.org/10.1016/0009-3084(89)90054-6
State	Published - Jun 1989

Keywords

dipole
evanescent
microscopy
near and asymptotic fields
order parameters
orientation distribution
radiation

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Organic Chemistry
Cell Biology

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10.1016/0009-3084(89)90054-6

Cite this

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title = "Polarized fluorescent emission from probes near dielectric interfaces",

abstract = "The electromagnetic field surrounding and emitted by a dipolar molecular probe very near to a dielectric interface is the sum of the real dipole field and the field of the image dipole induced inside the dielectric interface. The total charge distribution, made up of the real and image dipoles in close proximity to each other, approximates a quadrupole distribution and emits a light intensity pattern similar to that of an oscillating electric quadrupole. The electromagnetic field emitted by this system contains information that can be directly related to the spatial and orientational distributions of the dipole near the interface. Experimental methods are discussed that utilize this system for determining the spatial and orientational distribution of fluorescent probes in biological material.",

keywords = "dipole, evanescent, microscopy, near and asymptotic fields, order parameters, orientation distribution, radiation",

author = "Burghardt, {Thomas P.}",

note = "Funding Information: The writing of this paper was supported by a grant from the Mayo Foundation. Computer time was obtained from the Research Computer Facility of the Mayo Foundation. T.P.B. is an Established Investigator of the American Heart Association.",

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T1 - Polarized fluorescent emission from probes near dielectric interfaces

AU - Burghardt, Thomas P.

N1 - Funding Information: The writing of this paper was supported by a grant from the Mayo Foundation. Computer time was obtained from the Research Computer Facility of the Mayo Foundation. T.P.B. is an Established Investigator of the American Heart Association.

PY - 1989/6

Y1 - 1989/6

N2 - The electromagnetic field surrounding and emitted by a dipolar molecular probe very near to a dielectric interface is the sum of the real dipole field and the field of the image dipole induced inside the dielectric interface. The total charge distribution, made up of the real and image dipoles in close proximity to each other, approximates a quadrupole distribution and emits a light intensity pattern similar to that of an oscillating electric quadrupole. The electromagnetic field emitted by this system contains information that can be directly related to the spatial and orientational distributions of the dipole near the interface. Experimental methods are discussed that utilize this system for determining the spatial and orientational distribution of fluorescent probes in biological material.

AB - The electromagnetic field surrounding and emitted by a dipolar molecular probe very near to a dielectric interface is the sum of the real dipole field and the field of the image dipole induced inside the dielectric interface. The total charge distribution, made up of the real and image dipoles in close proximity to each other, approximates a quadrupole distribution and emits a light intensity pattern similar to that of an oscillating electric quadrupole. The electromagnetic field emitted by this system contains information that can be directly related to the spatial and orientational distributions of the dipole near the interface. Experimental methods are discussed that utilize this system for determining the spatial and orientational distribution of fluorescent probes in biological material.

KW - dipole

KW - evanescent

KW - microscopy

KW - near and asymptotic fields

KW - order parameters

KW - orientation distribution

KW - radiation

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JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

IS - 3-4

ER -

Polarized fluorescent emission from probes near dielectric interfaces

Abstract

Keywords

ASJC Scopus subject areas

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