Pneumococcal serotype-specific cut-offs based on antibody responses to pneumococcal polysaccharide vaccination in healthy adults

Miguel A. Park, Sarah M. Jenkins, Carin Y. Smith, Regan C. Pyle, Keith A. Sacco, Euijung Ryu, John B. Hagan, Avni Y. Joshi, Melissa R. Snyder, Roshini S. Abraham

Research output: Contribution to journalArticlepeer-review


Antibody responses to pneumococcal polysaccharide vaccination are frequently used as a diagnostic tool for humoral immunodeficiencies, part of the larger collection of inborn errors of immunity. Currently, arbitrary criteria, such as a serotype specific titer of >/= 1.3 µg/mL is most often used as a cut-off for interpretation of pneumococcal antibody responses. The magnitude of the antibody response to each of the 23 serotypes in Pneumovax®, and serotype-specific cut-offs in healthy pneumococcal vaccine-naïve adults has not been previously characterized. IgG antibody concentrations were measured prospectively for 23 pneumococcal serotypes pre and 4–6 weeks post-Pneumovax® vaccination in 100 healthy adults, using a multiplex bead-based assay. Antibodies to 19 of 23 serotypes were informative for distinguishing subjects who responded to vaccination, and the serotype threshold was determined to be 9 of 19 serotypes, which characterized an antibody response to pneumococcal vaccination. While this study may facilitate classification of IgG serotype-specific antibody responses post-pneumococcal vaccination in adult patients undergoing diagnostic immunological evaluation for antibody immunodeficiencies or other relevant contexts, additional studies in healthy children and S. pneumoniae protein-conjugate-vaccinated healthy adults will need to be undertaken in the future.

Original languageEnglish (US)
Pages (from-to)2850-2856
Number of pages7
Issue number21
StatePublished - May 18 2021


  • Antibody response
  • Humoral immunodeficiency
  • Multiplex bead analysis
  • Pneumococcal
  • Pneumococcal conjugate vaccine
  • Pneumococcal polysaccharide vaccine
  • Pneumovax®
  • Prevnar®
  • Primary immunodeficiency
  • Serotype

ASJC Scopus subject areas

  • Molecular Medicine
  • General Immunology and Microbiology
  • General Veterinary
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases


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