TY - JOUR
T1 - Pharmacological activation changes stiffness of cultured human airway smooth muscle cells
AU - Hubmayr, Rolf D.
AU - Shore, Stephanie A.
AU - Fredberg, Jeffrey J.
AU - Planus, Emanuelle
AU - Panettieri, Reynold A.
AU - Moller, Winfried
AU - Heyder, Joachim
AU - Wang, Ning
PY - 1996/11
Y1 - 1996/11
N2 - Using magnetic twisting cytometry (MTC), we measured the cytoskeletal stiffness of adherent human airway smooth muscle (HASM) cells. We hypothesized that modulation of actin-myosin interactions by application of contractile agonists would induce changes in cytoskeletal stiffness. In cells plated on high-density collagen, bradykinin (10-6 M) and histamine (10-4 M) increased stiffness by 85 ± 15 and 68 ± 16%, respectively. Increases in cell stiffness were also consistently observed after acetylcholine, substance P, and KCl. The bronchodilator agonists isoproterenol, prostaglandin E2, forskolin, dibutryl adenosine 3',5'-cyclic monophosphate, and 8- bromoguanosine 3',5'-cyclic monophosphate each caused a dose-dependent decrease in cell stiffness in unstimulated as well as bradykinin-treated cells. HASM cells plated on high-density collagen were stiffer than cells plated on low-density collagen (126 ± 16 vs. 43 ± 3 dyn/cm2) and developed more pronounced increases in stiffness in response to bradykinin as well as more pronounced decreases in stiffness in response to isoproterenol. These results are consistent with the hypothesis that modulation of actin-myosin interactions by application of contractile agonists causes changes in cytoskeletal stiffness of HASM cells. MTC may be a valuable tool for evaluating the mechanisms of pharmacomechanical coupling in airway smooth muscle cells in culture.
AB - Using magnetic twisting cytometry (MTC), we measured the cytoskeletal stiffness of adherent human airway smooth muscle (HASM) cells. We hypothesized that modulation of actin-myosin interactions by application of contractile agonists would induce changes in cytoskeletal stiffness. In cells plated on high-density collagen, bradykinin (10-6 M) and histamine (10-4 M) increased stiffness by 85 ± 15 and 68 ± 16%, respectively. Increases in cell stiffness were also consistently observed after acetylcholine, substance P, and KCl. The bronchodilator agonists isoproterenol, prostaglandin E2, forskolin, dibutryl adenosine 3',5'-cyclic monophosphate, and 8- bromoguanosine 3',5'-cyclic monophosphate each caused a dose-dependent decrease in cell stiffness in unstimulated as well as bradykinin-treated cells. HASM cells plated on high-density collagen were stiffer than cells plated on low-density collagen (126 ± 16 vs. 43 ± 3 dyn/cm2) and developed more pronounced increases in stiffness in response to bradykinin as well as more pronounced decreases in stiffness in response to isoproterenol. These results are consistent with the hypothesis that modulation of actin-myosin interactions by application of contractile agonists causes changes in cytoskeletal stiffness of HASM cells. MTC may be a valuable tool for evaluating the mechanisms of pharmacomechanical coupling in airway smooth muscle cells in culture.
KW - cell mechanics
KW - cytoskeleton
KW - magnetic twisting cytometry
KW - signal transduction
KW - smooth muscle contraction
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U2 - 10.1152/ajpcell.1996.271.5.c1660
DO - 10.1152/ajpcell.1996.271.5.c1660
M3 - Article
C2 - 8944650
AN - SCOPUS:0029827044
SN - 0363-6143
VL - 271
SP - C1660-C1668
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 5 40-5
ER -