Abstract
We report the first successful collection and biochemical analysis of endoneurially enriched fluid (EEF) from sciatic nerve of control and lead-intoxicated rats using an implanted polyethylene matrix capsule and collector. The EEF protein concentration was approximately one-half that of rat serum. Protein fractionation by sodium dodecyl sulfate-pore gradient electrophoresis revealed a different protein composition for the endoneurium, EEF, and serum from the same animal. In EEF there is a complex array of protein bands with a molecular weight range of 104 to 2 × 105 of which albumin was a major constituent. Preliminary results on lead-intoxicated rats suggest that total endoneurial fluid protein may be increased during lead intoxication.
Original language | English (US) |
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Pages (from-to) | 208-214 |
Number of pages | 7 |
Journal | Experimental Neurology |
Volume | 77 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1982 |
ASJC Scopus subject areas
- Neurology
- Developmental Neuroscience