TY - JOUR
T1 - Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells
AU - Clark, Karl J.
AU - Carlson, Daniel F.
AU - Leaver, Michael J.
AU - Foster, Linda K.
AU - Fahrenkrug, Scott C.
N1 - Funding Information:
This work and funding for open access publication was supported by grants from the USDA National Research Initiative (2008-35205-18852 to S.C.F.).
PY - 2009
Y1 - 2009
N2 - The Tc1/mariner family of DNA transposons is widespread across fungal, plant and animal kingdoms, and thought to contribute to the evolution of their host genomes. To date, an active Tc1 transposon has not been identified within the native genome of a vertebrate. We demonstrate that Passport, a native transposon isolated from a fish (Pleuronectes platessa), is active in a variety of vertebrate cells. In transposition assays, we found that the Passport transposon system improved stable cellular transgenesis by 40-fold, has an apparent preference for insertion into genes, and is subject to overproduction inhibition like other Tc1 elements. Passport represents the first vertebrate Tc1 element described as both natively intact and functionally active, and given its restricted phylogenetic distribution, may be contemporaneously active. The Passport transposon system thus complements the available genetic tools for the manipulation of vertebrate genomes, and may provide a unique system for studying the infiltration of vertebrate genomes by Tc1 elements.
AB - The Tc1/mariner family of DNA transposons is widespread across fungal, plant and animal kingdoms, and thought to contribute to the evolution of their host genomes. To date, an active Tc1 transposon has not been identified within the native genome of a vertebrate. We demonstrate that Passport, a native transposon isolated from a fish (Pleuronectes platessa), is active in a variety of vertebrate cells. In transposition assays, we found that the Passport transposon system improved stable cellular transgenesis by 40-fold, has an apparent preference for insertion into genes, and is subject to overproduction inhibition like other Tc1 elements. Passport represents the first vertebrate Tc1 element described as both natively intact and functionally active, and given its restricted phylogenetic distribution, may be contemporaneously active. The Passport transposon system thus complements the available genetic tools for the manipulation of vertebrate genomes, and may provide a unique system for studying the infiltration of vertebrate genomes by Tc1 elements.
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U2 - 10.1093/nar/gkn1025
DO - 10.1093/nar/gkn1025
M3 - Article
C2 - 19136468
AN - SCOPUS:62549101398
SN - 0305-1048
VL - 37
SP - 1239
EP - 1247
JO - Nucleic acids research
JF - Nucleic acids research
IS - 4
ER -