Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis

Hamish I. Stewart; Dmitry Grinfeld; Anastassios Giannakopulos; Johannes Petzoldt; Toby Shanley; Matthew Garland; Eduard Denisov; Amelia C. Peterson; Eugen Damoc; Martin Zeller; Tabiwang N. Arrey; Anna Pashkova; Santosh Renuse; Amirmansoor Hakimi; Andreas Kühn; Matthias Biel; Arne Kreutzmann; Bernd Hagedorn; Immo Colonius; Adrian Schütz; Arne Stefes; Ankit Dwivedi; Daniel Mourad; Max Hoek; Bastian Reitemeier; Philipp Cochems; Alexander Kholomeev; Robert Ostermann; Gregor Quiring; Maximilian Ochmann; Sascha Möhring; Alexander Wagner; André Petker; Sebastian Kanngiesser; Michael Wiedemeyer; Wilko Balschun; Daniel Hermanson; Vlad Zabrouskov; Alexander A. Makarov; Christian Hock

doi:10.1021/acs.analchem.3c02856

Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis

Hamish I. Stewart, Dmitry Grinfeld, Anastassios Giannakopulos, Johannes Petzoldt, Toby Shanley, Matthew Garland, Eduard Denisov, Amelia C. Peterson, Eugen Damoc, Martin Zeller, Tabiwang N. Arrey, Anna Pashkova, Santosh Renuse, Amirmansoor Hakimi, Andreas Kühn, Matthias Biel, Arne Kreutzmann, Bernd Hagedorn, Immo Colonius, Adrian SchützArne Stefes, Ankit Dwivedi, Daniel Mourad, Max Hoek, Bastian Reitemeier, Philipp Cochems, Alexander Kholomeev, Robert Ostermann, Gregor Quiring, Maximilian Ochmann, Sascha Möhring, Alexander Wagner, André Petker, Sebastian Kanngiesser, Michael Wiedemeyer, Wilko Balschun, Daniel Hermanson, Vlad Zabrouskov, Alexander A. Makarov, Christian Hock

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather the large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time and necessarily sacrifice sensitivity and resolving power to deliver higher acquisition rates. We developed a new mass spectrometer that combines a mass-resolving quadrupole, the Orbitrap, and the novel Asymmetric Track Lossless (Astral) analyzer. The new hybrid instrument enables faster acquisition of high-resolution accurate mass (HRAM) MS/MS spectra compared with state-of-the-art mass spectrometers. Accordingly, new proteomics methods were developed that leverage the strengths of each HRAM analyzer, whereby the Orbitrap analyzer performs full scans with a high dynamic range and resolution, synchronized with the Astral analyzer’s acquisition of fast and sensitive HRAM MS/MS scans.

Original language	English (US)
Pages (from-to)	15656-15664
Number of pages	9
Journal	Analytical Chemistry
Volume	95
Issue number	42
DOIs	https://doi.org/10.1021/acs.analchem.3c02856
State	Published - Oct 24 2023

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/acs.analchem.3c02856

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Stewart, H. I., Grinfeld, D., Giannakopulos, A., Petzoldt, J., Shanley, T., Garland, M., Denisov, E., Peterson, A. C., Damoc, E., Zeller, M., Arrey, T. N., Pashkova, A., Renuse, S., Hakimi, A., Kühn, A., Biel, M., Kreutzmann, A., Hagedorn, B., Colonius, I., ... Hock, C. (2023). Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis. Analytical Chemistry, 95(42), 15656-15664. https://doi.org/10.1021/acs.analchem.3c02856

Stewart, HI, Grinfeld, D, Giannakopulos, A, Petzoldt, J, Shanley, T, Garland, M, Denisov, E, Peterson, AC, Damoc, E, Zeller, M, Arrey, TN, Pashkova, A, Renuse, S, Hakimi, A, Kühn, A, Biel, M, Kreutzmann, A, Hagedorn, B, Colonius, I, Schütz, A, Stefes, A, Dwivedi, A, Mourad, D, Hoek, M, Reitemeier, B, Cochems, P, Kholomeev, A, Ostermann, R, Quiring, G, Ochmann, M, Möhring, S, Wagner, A, Petker, A, Kanngiesser, S, Wiedemeyer, M, Balschun, W, Hermanson, D, Zabrouskov, V, Makarov, AA & Hock, C 2023, 'Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis', Analytical Chemistry, vol. 95, no. 42, pp. 15656-15664. https://doi.org/10.1021/acs.analchem.3c02856

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title = "Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis",

abstract = "The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather the large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time and necessarily sacrifice sensitivity and resolving power to deliver higher acquisition rates. We developed a new mass spectrometer that combines a mass-resolving quadrupole, the Orbitrap, and the novel Asymmetric Track Lossless (Astral) analyzer. The new hybrid instrument enables faster acquisition of high-resolution accurate mass (HRAM) MS/MS spectra compared with state-of-the-art mass spectrometers. Accordingly, new proteomics methods were developed that leverage the strengths of each HRAM analyzer, whereby the Orbitrap analyzer performs full scans with a high dynamic range and resolution, synchronized with the Astral analyzer{\textquoteright}s acquisition of fast and sensitive HRAM MS/MS scans.",

author = "Stewart, {Hamish I.} and Dmitry Grinfeld and Anastassios Giannakopulos and Johannes Petzoldt and Toby Shanley and Matthew Garland and Eduard Denisov and Peterson, {Amelia C.} and Eugen Damoc and Martin Zeller and Arrey, {Tabiwang N.} and Anna Pashkova and Santosh Renuse and Amirmansoor Hakimi and Andreas K{\"u}hn and Matthias Biel and Arne Kreutzmann and Bernd Hagedorn and Immo Colonius and Adrian Sch{\"u}tz and Arne Stefes and Ankit Dwivedi and Daniel Mourad and Max Hoek and Bastian Reitemeier and Philipp Cochems and Alexander Kholomeev and Robert Ostermann and Gregor Quiring and Maximilian Ochmann and Sascha M{\"o}hring and Alexander Wagner and Andr{\'e} Petker and Sebastian Kanngiesser and Michael Wiedemeyer and Wilko Balschun and Daniel Hermanson and Vlad Zabrouskov and Makarov, {Alexander A.} and Christian Hock",

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year = "2023",

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doi = "10.1021/acs.analchem.3c02856",

language = "English (US)",

volume = "95",

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T1 - Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis

AU - Stewart, Hamish I.

AU - Grinfeld, Dmitry

AU - Giannakopulos, Anastassios

AU - Petzoldt, Johannes

AU - Shanley, Toby

AU - Garland, Matthew

AU - Denisov, Eduard

AU - Peterson, Amelia C.

AU - Damoc, Eugen

AU - Zeller, Martin

AU - Arrey, Tabiwang N.

AU - Pashkova, Anna

AU - Renuse, Santosh

AU - Hakimi, Amirmansoor

AU - Kühn, Andreas

AU - Biel, Matthias

AU - Kreutzmann, Arne

AU - Hagedorn, Bernd

AU - Colonius, Immo

AU - Schütz, Adrian

AU - Stefes, Arne

AU - Dwivedi, Ankit

AU - Mourad, Daniel

AU - Hoek, Max

AU - Reitemeier, Bastian

AU - Cochems, Philipp

AU - Kholomeev, Alexander

AU - Ostermann, Robert

AU - Quiring, Gregor

AU - Ochmann, Maximilian

AU - Möhring, Sascha

AU - Wagner, Alexander

AU - Petker, André

AU - Kanngiesser, Sebastian

AU - Wiedemeyer, Michael

AU - Balschun, Wilko

AU - Hermanson, Daniel

AU - Zabrouskov, Vlad

AU - Makarov, Alexander A.

AU - Hock, Christian

PY - 2023/10/24

Y1 - 2023/10/24

N2 - The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather the large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time and necessarily sacrifice sensitivity and resolving power to deliver higher acquisition rates. We developed a new mass spectrometer that combines a mass-resolving quadrupole, the Orbitrap, and the novel Asymmetric Track Lossless (Astral) analyzer. The new hybrid instrument enables faster acquisition of high-resolution accurate mass (HRAM) MS/MS spectra compared with state-of-the-art mass spectrometers. Accordingly, new proteomics methods were developed that leverage the strengths of each HRAM analyzer, whereby the Orbitrap analyzer performs full scans with a high dynamic range and resolution, synchronized with the Astral analyzer’s acquisition of fast and sensitive HRAM MS/MS scans.

AB - The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather the large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time and necessarily sacrifice sensitivity and resolving power to deliver higher acquisition rates. We developed a new mass spectrometer that combines a mass-resolving quadrupole, the Orbitrap, and the novel Asymmetric Track Lossless (Astral) analyzer. The new hybrid instrument enables faster acquisition of high-resolution accurate mass (HRAM) MS/MS spectra compared with state-of-the-art mass spectrometers. Accordingly, new proteomics methods were developed that leverage the strengths of each HRAM analyzer, whereby the Orbitrap analyzer performs full scans with a high dynamic range and resolution, synchronized with the Astral analyzer’s acquisition of fast and sensitive HRAM MS/MS scans.

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DO - 10.1021/acs.analchem.3c02856

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SP - 15656

EP - 15664

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 42

ER -

Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis

Abstract

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