TY - JOUR
T1 - Organ-specific regulation of pro-inflammatory molecules in heart, lung, and kidney following brain death
AU - Skrabal, Christian A.
AU - Thompson, Larry O.
AU - Potapov, Evgenji V.
AU - Southard, Robert E.
AU - Joyce, David L.
AU - Youker, Keith A.
AU - Noon, George P.
AU - Loebe, Matthias
N1 - Funding Information:
Dr. Skrabal was supported by a grant from the German Research Foundation (DFG, SK 65/1-1)
PY - 2005/1
Y1 - 2005/1
N2 - Nonspecific inflammatory events following brain death may increase the intensity of the immunological host response. The present study investigated the course of pro-inflammatory molecules in heart, lung, kidney, and plasma after brain death induction. Brain death was induced in five pigs by inflation of an intracranial Foley catheter and five pigs were sham-operated as controls. Each experiment was terminated 6 h after brain death/sham operation and the organs were harvested. We measured the mRNA and protein levels for TNF-α, IL-1β, and IL-6 in heart, lung, kidney, and plasma. Additionally, the mRNA expression for IL-6R, ICAM-1, MCP-1, and TGF-β was determined in each organ. After 6 h, the plasma cytokine levels were higher in the brain-dead animals than in the sham-operated. In heart, lung, and kidney there was an increase in IL-6 and IL-1β following brain death, while TNF-α was up-regulated in lung only (P < 0.05). MCP-1 and TGF-β were significantly higher in heart and lung and IL-6R increased in heart after brain death (P < 0.05). Brain death was associated with non-uniform cytokine expression patterns in the investigated organs. These expression patterns may cause variable pro-inflammatory priming resulting in different degrees of damage and explain the organ-specific variation in outcomes after transplantations.
AB - Nonspecific inflammatory events following brain death may increase the intensity of the immunological host response. The present study investigated the course of pro-inflammatory molecules in heart, lung, kidney, and plasma after brain death induction. Brain death was induced in five pigs by inflation of an intracranial Foley catheter and five pigs were sham-operated as controls. Each experiment was terminated 6 h after brain death/sham operation and the organs were harvested. We measured the mRNA and protein levels for TNF-α, IL-1β, and IL-6 in heart, lung, kidney, and plasma. Additionally, the mRNA expression for IL-6R, ICAM-1, MCP-1, and TGF-β was determined in each organ. After 6 h, the plasma cytokine levels were higher in the brain-dead animals than in the sham-operated. In heart, lung, and kidney there was an increase in IL-6 and IL-1β following brain death, while TNF-α was up-regulated in lung only (P < 0.05). MCP-1 and TGF-β were significantly higher in heart and lung and IL-6R increased in heart after brain death (P < 0.05). Brain death was associated with non-uniform cytokine expression patterns in the investigated organs. These expression patterns may cause variable pro-inflammatory priming resulting in different degrees of damage and explain the organ-specific variation in outcomes after transplantations.
KW - adhesion molecules
KW - brain death
KW - chemoattractant molecules
KW - cytokines
KW - donor organs
KW - inflammation
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U2 - 10.1016/j.jss.2004.07.245
DO - 10.1016/j.jss.2004.07.245
M3 - Article
C2 - 15652959
AN - SCOPUS:11844268064
SN - 0022-4804
VL - 123
SP - 118
EP - 125
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -