NY-ESO-1 protein glycosylated by yeast induces enhanced immune responses

Andreas Wadle, Axel Mischo, Sabine Strahl, Hiroyoshi Nishikawa, Gerhard Held, Frank Neumann, Beate Wullner, Eliane Fischer, Sascha Kleber, Julia Karbach, Elke Jager, Hiroshi Shiku, Kunle Odunsi, Protul A. Shrikant, Alexander Knuth, Vincenzo Cerundolo, Christoph Renner

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. Here we report that the genetically engineered yeast Saccharomyces cerevisiae, expressing the full-length tumour-associated antigen NY-ESO-1, is a versatile host for protein production. Exposing dendritic cells (DCs) to soluble NY-ESO-1 protein linked to the yeast a-agglutinin 2 protein (Aga2p) protein resulted in protein uptake, processing and MHC class I cross-presentation of NY-ESO-1-derived peptides. The process of antigen uptake and cross-presentation was dependent on the glycosylation pattern of NY-ESO-1-Aga2p protein and the presence of accessible mannose receptors. In addition, NY-ESO-1-Aga2p protein uptake by dendritic cells resulted in recognition by HLA-DP4 NY-ESO-1-specific CD4+ T cells, indicating MHC class II presentation. Finally, vaccination of mice with yeast-derived NY-ESO-1-Aga2p protein led to an enhanced humoral and cellular immune response, when compared to the bacterially expressed NY-ESO-1 protein. Together, these data demonstrate that yeast-derived full-length NY-ESO-1-Aga2p protein is processed and presented efficiently by MHC class I and II complexes and warrants clinical trials to determine the potential value of S. cerevisiae as a host for cancer vaccine development.

Original languageEnglish (US)
Pages (from-to)919-931
Number of pages13
Issue number11
StatePublished - Nov 2010


  • Cross-presentation
  • Cytotoxic T cells
  • Glycosylation
  • MHC
  • Peptide processing
  • Presentation
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics


Dive into the research topics of 'NY-ESO-1 protein glycosylated by yeast induces enhanced immune responses'. Together they form a unique fingerprint.

Cite this