@article{ccece4c4c95b40cea6da88244eccc348,
title = "Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1",
abstract = "We previously identified lynx1 as a neuronal membrane molecule related to snake α-neurotoxins able to modulate nAChRs. Here, we show that lynx1 colocalizes with nAChRs on CNS neurons and physically associates with nAChRs. Single-channel recordings show that lynx1 promotes the largest of three current amplitudes elicited by ACh through α4β2 nAChRs and that lynx1 enhances desensitization. Macroscopic recordings quantify the enhancement of desensitization onset by lynx1 and further show that it slows recovery from desensitization and increases the EC50. These experiments establish that direct interaction of lynx1 with nAChRs can result in a novel type of functional modulation and suggest that prototoxins may play important roles in vivo by modulating functional properties of their cognate CNS receptors.",
author = "In{\'e}s Iba{\~n}ez-Tallon and Miwa, {Julie M.} and Wang, {Hai Long} and Adams, {Niels C.} and Crabtree, {Gregg W.} and Sine, {Steven M.} and Nathaniel Heintz",
note = "Funding Information: We thank Scott Rogers for the rabbit polyclonal antibody against mouse α 4 nAChRs; Marc Ballivet, Jos{\'e} Ramirez-LaTorre, and Myles Akabas for the expression plasmids encoding nAChR and GABA receptor subunit cDNAs; and Zhenyu Yue and Toshifumi Tomoda for the Grid and PLAP expression plasmids, respectively. We are grateful to Lorna W. Role for her scientific expertise and advice during the course of this study and her thoughtful comments on the manuscript. We would like to thank Paola Vergani for her help with the capacitance measurements, and together with Pablo Artigas, Lain F. Diaz, and David C. Gadsby for their expert advice and valuable discussions. We would like to acknowledge Daniel Besser for critical reading of the manuscript and helpful discussions and Ali Hemmati-Brivanlou, Chenbei B. Chang, and Kathy Zimmerman for help with the injection of Xenopus oocytes. This research was supported by the Howard Hughes Medical Institute, NIH NINDS P01 NS30532; AT Children's Project and HHMI (I.I.-T.); The Rockefeller University and NIH CA 09673 (J.M.M.); NS-22061 (to L.W. Role for support of G.W.C.) and NS-31744 (S.M.S.).",
year = "2002",
month = mar,
day = "14",
doi = "10.1016/S0896-6273(02)00632-3",
language = "English (US)",
volume = "33",
pages = "893--903",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",
}