Novel FLT3 point mutations within exon 14 found in patients with acute myeloid leukaemia

Derek L. Stirewalt, Soheil Meshinchi, Steven J. Kussick, Kayla M. Sheets, Era Pogosova-Agadjanyan, Cheryl L. Willman, Jerald P. Radich

Research output: Contribution to journalArticlepeer-review


Internal tandem duplications in FLT3 are the most common mutation in acute myeloid leukaemia (AML), with agarose gel electrophoresis of polymerase chain reaction products (PCR/agarose) being the screening method of choice for these mutations. As PCR/agarose screening does not detect small mutations, single-stranded conformational polymorphism analyses (PCR/SSCP) were used in an attempt to identify previously unrecognized point mutations in FLT3 exons 14 and 15 of 140 AML patients, using newly designed primers that anneal within intron sequences. Novel missense point mutations were found in exon 14, suggesting additional investigations should be performed in AML and other haematopoietic malignancies, using this sensitive technique.

Original languageEnglish (US)
Pages (from-to)481-484
Number of pages4
JournalBritish journal of haematology
Issue number4
StatePublished - Feb 2004


  • Acute myeloid leukaemia
  • FLT3
  • Mutation
  • Tyrosine kinase receptor

ASJC Scopus subject areas

  • Hematology


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