TY - JOUR
T1 - Non-random genetic alterations in the cyanobacterium Nostoc sp. exposed to space conditions
AU - Liu, Yuguang
AU - Jeraldo, Patricio
AU - Herbert, William
AU - McDonough, Samantha
AU - Eckloff, Bruce
AU - de Vera, Jean Pierre
AU - Cockell, Charles
AU - Leya, Thomas
AU - Baqué, Mickael
AU - Jen, Jin
AU - Schulze-Makuch, Dirk
AU - Walther-Antonio, Marina
N1 - Funding Information:
This research was funded by the following sources: Marina Walther-Antonio and Yuguang Liu acknowledge The Ivan Bowen Family Foundation. This project was supported by CTSA Grant Number KL2TR002379 from the National Center for Advancing Translational Science (NCATS). Contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Dirk Schulze-Makuch and Jean-Pierre de Vera acknowledge support from ERC Advanced Grant “HOME” (# 339231), and in addition, Jean-Pierre de Vera acknowledges support from ESA for the BIOMEX project (ESA-ILSRA 2009-0834) and DLR for the DLR-FuW-Project BIOMEX (2474128). Mickael Baqué acknowledges the support of the Deutsche Forschungsgemeinschaft (DFG—German Research Foundation) for the project “Raman Biosignatures for Astrobiology Research” (RaBioFAM; project number: 426601242). Charles S. Cockell was supported by the Science and Technology Facilities Council (STFC), Grant No. ST/M001261/1. In addition, we thank the Microbiome Program and the Center for Individualized Medicine at Mayo Clinic for their support, and Dr. Alexander Revzin at Mayo Clinic for granting us the access to his microfabrication facilities.
Funding Information:
This research was funded by the following sources: Marina Walther-Antonio and Yuguang Liu acknowledge The Ivan Bowen Family Foundation. This project was supported by CTSA Grant Number KL2TR002379 from the National Center for Advancing Translational Science (NCATS). Contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Dirk Schulze-Makuch and Jean-Pierre de Vera acknowledge support from ERC Advanced Grant “HOME” (# 339231), and in addition, Jean-Pierre de Vera acknowledges support from ESA for the BIOMEX project (ESA-ILSRA 2009-0834) and DLR for the DLR-FuW-Project BIOMEX (2474128). Mickael Baqué acknowledges the support of the Deutsche Forschungsgemeinschaft (DFG—German Research Foundation) for the project “Raman Biosignatures for Astrobiology Research” (RaBioFAM; project number: 426601242). Charles S. Cockell was supported by the Science and Technology Facilities Council (STFC), Grant No. ST/M001261/1. In addition, we thank the Microbiome Program and the Center for Individualized Medicine at Mayo Clinic for their support, and Dr. Alexander Revzin at Mayo Clinic for granting us the access to his microfabrication facilities.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Understanding the impact of long-term exposure of microorganisms to space is critical in understanding how these exposures impact the evolution and adaptation of microbial life under space conditions. In this work we subjected Nostoc sp. CCCryo 231-06, a cyanobacterium capable of living under many different ecological conditions, and also surviving in extreme ones, to a 23-month stay at the International Space Station (the Biology and Mars Experiment, BIOMEX, on the EXPOSE-R2 platform) and returned it to Earth for single-cell genome analysis. We used microfluidic technology and single cell sequencing to identify the changes that occurred in the whole genome of single Nostoc cells. The variant profile showed that biofilm and photosystem associated loci were the most altered, with an increased variant rate of synonymous base pair substitutions. The cause(s) of these non-random alterations and their implications to the evolutionary potential of single bacterial cells under long-term cosmic exposure warrants further investigation.
AB - Understanding the impact of long-term exposure of microorganisms to space is critical in understanding how these exposures impact the evolution and adaptation of microbial life under space conditions. In this work we subjected Nostoc sp. CCCryo 231-06, a cyanobacterium capable of living under many different ecological conditions, and also surviving in extreme ones, to a 23-month stay at the International Space Station (the Biology and Mars Experiment, BIOMEX, on the EXPOSE-R2 platform) and returned it to Earth for single-cell genome analysis. We used microfluidic technology and single cell sequencing to identify the changes that occurred in the whole genome of single Nostoc cells. The variant profile showed that biofilm and photosystem associated loci were the most altered, with an increased variant rate of synonymous base pair substitutions. The cause(s) of these non-random alterations and their implications to the evolutionary potential of single bacterial cells under long-term cosmic exposure warrants further investigation.
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U2 - 10.1038/s41598-022-16789-w
DO - 10.1038/s41598-022-16789-w
M3 - Article
AN - SCOPUS:85134576108
SN - 2045-2322
VL - 12
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 12580
ER -