NMR microscopy of tissue in organic and mixed solvents

Slobodan Macura; Prasanna K. Mishra; Jeffrey D. Gamez; Istvan Pirko

doi:10.2298/JSC131108108M

NMR microscopy of tissue in organic and mixed solvents

Slobodan Macura, Prasanna K. Mishra, Jeffrey D. Gamez, Istvan Pirko

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The use organic and mixed solvents for nuclear magnetic resonance microscopy of fixed tissue is proposed as a means for improving image information content. NMR properties of some standard solvents (methanol, acetone or DMSO) and solvents in use for tissue processing in pathology (xylenes, paraffin, "Clearify") have been measured, reviewed and analyzed. It was found that DMSO and paraffin are very useful solvents that provide images of better quality than those obtained in water (neutralized formalin buffer). This is illustrated on formalin-fixed mouse brain sections imaged at 16.4 T (700 MHz).

Original language	English (US)
Pages (from-to)	1641-1654
Number of pages	14
Journal	Journal of the Serbian Chemical Society
Volume	78
Issue number	11
DOIs	https://doi.org/10.2298/JSC131108108M
State	Published - 2013

Keywords

Cross-relaxation
MRI
MRM
Magnetization transfer
NMR
NMR imaging
NMR microscopy
Nuclear magnetic resonance

ASJC Scopus subject areas

General Chemistry

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10.2298/JSC131108108M

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title = "NMR microscopy of tissue in organic and mixed solvents",

abstract = "The use organic and mixed solvents for nuclear magnetic resonance microscopy of fixed tissue is proposed as a means for improving image information content. NMR properties of some standard solvents (methanol, acetone or DMSO) and solvents in use for tissue processing in pathology (xylenes, paraffin, {"}Clearify{"}) have been measured, reviewed and analyzed. It was found that DMSO and paraffin are very useful solvents that provide images of better quality than those obtained in water (neutralized formalin buffer). This is illustrated on formalin-fixed mouse brain sections imaged at 16.4 T (700 MHz).",

keywords = "Cross-relaxation, MRI, MRM, Magnetization transfer, NMR, NMR imaging, NMR microscopy, Nuclear magnetic resonance",

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T1 - NMR microscopy of tissue in organic and mixed solvents

AU - Macura, Slobodan

AU - Mishra, Prasanna K.

AU - Gamez, Jeffrey D.

AU - Pirko, Istvan

PY - 2013

Y1 - 2013

N2 - The use organic and mixed solvents for nuclear magnetic resonance microscopy of fixed tissue is proposed as a means for improving image information content. NMR properties of some standard solvents (methanol, acetone or DMSO) and solvents in use for tissue processing in pathology (xylenes, paraffin, "Clearify") have been measured, reviewed and analyzed. It was found that DMSO and paraffin are very useful solvents that provide images of better quality than those obtained in water (neutralized formalin buffer). This is illustrated on formalin-fixed mouse brain sections imaged at 16.4 T (700 MHz).

AB - The use organic and mixed solvents for nuclear magnetic resonance microscopy of fixed tissue is proposed as a means for improving image information content. NMR properties of some standard solvents (methanol, acetone or DMSO) and solvents in use for tissue processing in pathology (xylenes, paraffin, "Clearify") have been measured, reviewed and analyzed. It was found that DMSO and paraffin are very useful solvents that provide images of better quality than those obtained in water (neutralized formalin buffer). This is illustrated on formalin-fixed mouse brain sections imaged at 16.4 T (700 MHz).

KW - Cross-relaxation

KW - MRI

KW - MRM

KW - Magnetization transfer

KW - NMR

KW - NMR imaging

KW - NMR microscopy

KW - Nuclear magnetic resonance

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NMR microscopy of tissue in organic and mixed solvents

Abstract

Keywords

ASJC Scopus subject areas

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