Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices

K. M. Schak, S. P. Scordilis, G. A. Ferreyra, M. E. Harrington

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The mammalian circadian clock in the suprachiasmatic nuclei (SCN) can be phase-shifted by neuropeptide Y applied in the subjective day. Previous studies suggested that neuropeptide Y might act through a protein kinase C (PKC)-dependent mechanism. We directly measured PKC activity in suprachiasmatic nuclei brain slices following application of neuropeptide Y. PKC activity increased 5 min after neuropeptide Y application, with a return to baseline levels 15 min after application. An initial small decrease in PKC activity 1 min after neuropeptide Y application was also observed after control applications of artificial cerebrospinal fluid. Our results support the hypothesis that phase shifts induced by neuropeptide Y involve activation of PKC.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalBiological Rhythm Research
Issue number2
StatePublished - 2001


  • Circadian
  • Neuropeptide Y
  • Protein kinase C assay
  • Rhythm
  • Suprachiasmatic

ASJC Scopus subject areas

  • Physiology (medical)
  • Ecology, Evolution, Behavior and Systematics
  • Physiology


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