TY - JOUR
T1 - Nervous system-specific expression of a novel serine protease
T2 - Regulation in the adult rat spinal cord by excitotoxic injury
AU - Scarisbrick, Isobel A.
AU - Towner, Melvin D.
AU - Isackson, Paul J.
PY - 1997
Y1 - 1997
N2 - A full-length cDNA clone of a previously unidentified serine protease, myelencephalon-specific protease (MSP), has been isolated by using a PCR cloning strategy and has been shown to be expressed in a nervous system and spinal cord-specific pattern. Sequence analysis demonstrated that MSP is most similar in sequence to neuropsin, trypsin, and tissue kallikrein and is predicted to have trypsin-like substrate specificity. MSP mRNA was found to be ~10-fold greater in the CNS of the rat and human, as compared with most peripheral tissues, and within the CNS was found to be highest by a factor of four in the medulla oblongata and spinal cord. Levels of mRNA encoding tissue plasminogen activator (tPA) also were elevated in the spinal cord but were more widespread in peripheral tissues as compared with MSR. In the adult rat lumbosacral spinal cord, in situ localization of MSP mRNA demonstrated 2- fold higher levels in the white, as compared with the gray, matter. MSP mRNA expression was shown to increase 3-fold in the white matter and 1.5-fold in the gray laminae at 72 hr after intraperitoneal injection of the AMPA/kainate glutamate receptor-specific agonist, kainic acid (KA). MSP mRNA remained elevated in the ventral gray matter, including expression associated with the motor neurons of lamina IX, at 7 d after the initial excitotoxic insult. Together, these observations indicate that MSP is in a position to play a fundamental role in normal homeostasis and in the response of the spinal cord to injury.
AB - A full-length cDNA clone of a previously unidentified serine protease, myelencephalon-specific protease (MSP), has been isolated by using a PCR cloning strategy and has been shown to be expressed in a nervous system and spinal cord-specific pattern. Sequence analysis demonstrated that MSP is most similar in sequence to neuropsin, trypsin, and tissue kallikrein and is predicted to have trypsin-like substrate specificity. MSP mRNA was found to be ~10-fold greater in the CNS of the rat and human, as compared with most peripheral tissues, and within the CNS was found to be highest by a factor of four in the medulla oblongata and spinal cord. Levels of mRNA encoding tissue plasminogen activator (tPA) also were elevated in the spinal cord but were more widespread in peripheral tissues as compared with MSR. In the adult rat lumbosacral spinal cord, in situ localization of MSP mRNA demonstrated 2- fold higher levels in the white, as compared with the gray, matter. MSP mRNA expression was shown to increase 3-fold in the white matter and 1.5-fold in the gray laminae at 72 hr after intraperitoneal injection of the AMPA/kainate glutamate receptor-specific agonist, kainic acid (KA). MSP mRNA remained elevated in the ventral gray matter, including expression associated with the motor neurons of lamina IX, at 7 d after the initial excitotoxic insult. Together, these observations indicate that MSP is in a position to play a fundamental role in normal homeostasis and in the response of the spinal cord to injury.
KW - Brain stem
KW - CNS
KW - Excitotoxicity
KW - Kainic acid
KW - Medulla oblongata
KW - Motor neuron
KW - Oligodendrocyte
KW - Serine protease
KW - Spinal cord
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U2 - 10.1523/jneurosci.17-21-08156.1997
DO - 10.1523/jneurosci.17-21-08156.1997
M3 - Article
C2 - 9334391
AN - SCOPUS:0030730261
SN - 0270-6474
VL - 17
SP - 8156
EP - 8168
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 21
ER -