Neoantigenic Potential of Complex Chromosomal Rearrangements in Mesothelioma

Aaron S. Mansfield, Tobias Peikert, James B. Smadbeck, Julia B.M. Udell, Enrique Garcia-Rivera, Laura Elsbernd, Courtney L. Erskine, Virginia P. Van Keulen, Farhad Kosari, Stephen J. Murphy, Hongzheng Ren, Vishnu V. Serla, Janet L. Schaefer Klein, Giannoula Karagouga, Faye R. Harris, Carlos Sosa, Sarah H. Johnson, Wendy Nevala, Svetomir N. Markovic, Aaron O. BungumEric S. Edell, Haidong Dong, John C. Cheville, Marie Christine Aubry, Jin Jen, George Vasmatzis

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Introduction: Malignant pleural mesothelioma is a disease primarily associated with exposure to the carcinogen asbestos. Whereas other carcinogen-related tumors are associated with a high tumor mutation burden, mesothelioma is not. We sought to resolve this discrepancy. Methods: We used mate-pair (n = 22), RNA (n = 28), and T cell receptor sequencing along with in silico predictions and immunologic assays to understand how structural variants of chromosomes affect the transcriptome. Results: We observed that inter- or intrachromosomal rearrangements were present in every specimen and were frequently in a pattern of chromoanagenesis such as chromoplexy or chromothripsis. Transcription of rearrangement-related junctions was predicted to result in many potential neoantigens, some of which were proven to bind patient-specific major histocompatibility complex molecules and to expand intratumoral T cell clones. T cells responsive to these predicted neoantigens were also present in a patient's circulating T cell repertoire. Analysis of genomic array data from the mesothelioma cohort in The Cancer Genome Atlas suggested that multiple chromothriptic-like events negatively impact survival. Conclusions: Our findings represent the discovery of potential neoantigen expression driven by structural chromosomal rearrangements. These results may have implications for the development of novel immunotherapeutic strategies and the selection of patients to receive immunotherapies.

Original languageEnglish (US)
Pages (from-to)276-287
Number of pages12
JournalJournal of Thoracic Oncology
Issue number2
StatePublished - Feb 2019


  • Chromoplexy
  • Chromosomal rearrangements
  • Chromothripsis
  • Mesothelioma
  • Neoantigens

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine


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