Multipotent menstrual blood stromal stem cells: Isolation, characterization, and differentiation

Amit N. Patel; Eulsoon Park; Michael Kuzman; Federico Benetti; Francisco J. Silva; Julie G. Allickson

doi:10.3727/096368908784153922

Multipotent menstrual blood stromal stem cells: Isolation, characterization, and differentiation

Amit N. Patel, Eulsoon Park, Michael Kuzman, Federico Benetti, Francisco J. Silva, Julie G. Allickson

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

The stromal stem cell fraction of many tissues and organs has demonstrated to exhibit stem cell properties such as the capability of self-renewal and multipotency, allowing for multilineage differentiation. In this study, we characterize a population of stromal stem cells derived from menstrual blood (MenSCs). We demonstrate that MenSCs are easily expandable to clinical relevance and express multipotent markers such as Oct-4, SSEA-4, and c-kit at the molecular and cellular level. Moreover, we demonstrate the multipotency of MenSCs by directionally differentiating MenSCs into chondrogenic, adipogenic, osteogenic, neurogenic, and cardiogenic cell lineages. These studies demonstrate the plasticity of MenSCs for potential research in regenerative medicine.

Original language	English (US)
Pages (from-to)	303-311
Number of pages	9
Journal	Cell transplantation
Volume	17
Issue number	3
DOIs	https://doi.org/10.3727/096368908784153922
State	Published - 2008

Keywords

Differentiation
Menstrual blood
Multipotent markers
Stromal stem cells

ASJC Scopus subject areas

Biomedical Engineering
Cell Biology
Transplantation

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10.3727/096368908784153922

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abstract = "The stromal stem cell fraction of many tissues and organs has demonstrated to exhibit stem cell properties such as the capability of self-renewal and multipotency, allowing for multilineage differentiation. In this study, we characterize a population of stromal stem cells derived from menstrual blood (MenSCs). We demonstrate that MenSCs are easily expandable to clinical relevance and express multipotent markers such as Oct-4, SSEA-4, and c-kit at the molecular and cellular level. Moreover, we demonstrate the multipotency of MenSCs by directionally differentiating MenSCs into chondrogenic, adipogenic, osteogenic, neurogenic, and cardiogenic cell lineages. These studies demonstrate the plasticity of MenSCs for potential research in regenerative medicine.",

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T2 - Isolation, characterization, and differentiation

AU - Patel, Amit N.

AU - Park, Eulsoon

AU - Kuzman, Michael

AU - Benetti, Federico

AU - Silva, Francisco J.

AU - Allickson, Julie G.

PY - 2008

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AB - The stromal stem cell fraction of many tissues and organs has demonstrated to exhibit stem cell properties such as the capability of self-renewal and multipotency, allowing for multilineage differentiation. In this study, we characterize a population of stromal stem cells derived from menstrual blood (MenSCs). We demonstrate that MenSCs are easily expandable to clinical relevance and express multipotent markers such as Oct-4, SSEA-4, and c-kit at the molecular and cellular level. Moreover, we demonstrate the multipotency of MenSCs by directionally differentiating MenSCs into chondrogenic, adipogenic, osteogenic, neurogenic, and cardiogenic cell lineages. These studies demonstrate the plasticity of MenSCs for potential research in regenerative medicine.

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KW - Menstrual blood

KW - Multipotent markers

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Multipotent menstrual blood stromal stem cells: Isolation, characterization, and differentiation

Abstract

Keywords

ASJC Scopus subject areas

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