Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound

Hussain Fatakdawala, Leigh G. Griffiths, Sterling Humphrey, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics XI
EditorsHyun Wook Kang, Justus F. Ilgner, Melissa C. Skala, Laura Marcu, Haishan Zeng, Mark W. Dewhirst, Kenton W. Gregory, Andreas Mandelis, Bernard Choi, Brian Jet-Fei Wong, Claus-Peter Richter, Guillermo J. Tearney, Alfred Nuttal, Nikiforos Kollias
ISBN (Electronic)9781628413939
StatePublished - 2015
EventPhotonic Therapeutics and Diagnostics XI - San Francisco, United States
Duration: Feb 7 2015Feb 8 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherPhotonic Therapeutics and Diagnostics XI
Country/TerritoryUnited States
CitySan Francisco


  • Time-resolved fluorescence
  • Tissue characterization
  • Tissue engineering
  • Ultrasound
  • Vascular grafts

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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