MRI Study of Jellyfish Collagen, Hyaluronic Acid, and Cadaveric Dermis for Injection Laryngoplasty

Serban San-Marina, Andrew J. Bowen, Michael S. Oldenburg, Stephen G. Voss, Danielle E. Hunter, Slobodan Macura, Ryan Meloche, Alen Lee Miller, Andrew Mearns Spragg, Dale C. Ekbom

Research output: Contribution to journalArticlepeer-review


Objectives/Hypothesis: Test a new jellyfish collagen biomaterial aimed to increase duration of injection medialization laryngoplasty (IL) against two products in clinical practice. Study Design: Animal model. Methods: Left recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits (N = 6/group). Group 1 received micronized cross-linked jellyfish collagen (MX-JC) and adipose derived stem cells (ADSCs), Group 2, MX-JC alone, Group 3, cross-linked hyaluronic acid (X-HA), and Group 4, micronized acellular dermis (MACD). Animals were sacrificed at 4 and 12 weeks. Major outcomes were MRI tissue volumes and histopathology. Results: After 100 μL IL MRI volumes (means ± STD) at 4 and 12 weeks were: Group 1: 27.2 ± 15.6 and 13.1 ± 5.2 μL, Group 2: 60.8 ± 18 and 27.8 ± 2.47 μL, Group 3: 27.4 ± 12 and 10.6 ± 8 μL, and Group 4: 37.5 ± 11 and 9.85 ± 1 μL. Group 2 volumes were largest and Group 3 were smallest in all comparisons (P <.05). Histologically, low grade inflammatory responses were observed in Group 1, mild histiocytic infiltration in Group 2, widespread muscle fiber loss in Group 3, and plasmocytic infiltration in Group 4. Conclusions: MX-JC showed the least resorption at 4 and 12 weeks among all groups. T cell inflammatory responses were observed with MX-JC but were reduced by 12 weeks while B cell immune responses, indicative of antibody priming, were predominantly noted with MACD. MX-JC + ADSC showed low grade immunity while the XHA showed greater myocyte loss compared to the other groups. Level of Evidence: NA Laryngoscope, 131:E2452–E2460, 2021.

Original languageEnglish (US)
Pages (from-to)E2452-E2460
Issue number8
StatePublished - Aug 2021


  • Cymetra®
  • Injection laryngoplasty
  • Jellyfish collagen
  • Restylane®
  • T-cell response
  • micronized acellular dermis
  • plasmocytic immunity
  • stem cells

ASJC Scopus subject areas

  • Otorhinolaryngology


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