@article{e4bd46243ead48948113597d6c4a9de5,
title = "Mouse oocytes depend on BubR1 for proper chromosome segregation but not for prophase i arrest",
abstract = "Mammalian female meiosis is error prone, with rates of meiotic chromosome missegregations strongly increasing towards the end of the reproductive lifespan. A strong reduction of BubR1 has been observed in oocytes of women approaching menopause and in ovaries of aged mice, which led to the hypothesis that a gradual decline of BubR1 contributes to age-related aneuploidization. Here we employ a conditional knockout approach in mouse oocytes to dissect the meiotic roles of BubR1. We show that BubR1 is required for diverse meiotic functions, including persistent spindle assembly checkpoint activity, timing of meiosis I and the establishment of robust kinetochore-microtubule attachments in a meiosis-specific manner, but not prophase I arrest. These data reveal that BubR1 plays a multifaceted role in chromosome segregation during the first meiotic division and suggest that age-related decline of BubR1 is a key determinant of the formation of aneuploid oocytes as women approach menopause.",
author = "Touati, {Sandra A.} and Eulalie Buffin and Damien Cladi{\`e}re and Khaled Hached and Christophe Rachez and {Van Deursen}, {Jan M.} and Katja Wassmann",
note = "Funding Information: We thank D. Baker (Mayo Clinic, MN, USA) for shipment of BubR1H/H mice, L. Malureanu (Mayo Clinic, MN, USA) for the murine BubR1 E406K mutant, S. Xie and P. Sorger (Havard Medical School, MA, USA) for the original Mps1F/F mouse strain, S. Taylor (The University of Manchester, Manchester, UK) for BubR1 antibody and T. Mayer (The University of Konstanz) for comments on the manuscript. We thank the following present or former lab members: S. Sin for help with statistical analysis, J. Rojas and A. Vallot for preliminary experiments, and W. El Yakoubi, I. Leontiou and I. Berenguer for discussions. We are grateful to administrative services (UMR7622), the cellular imaging facility of the IBPS, and to E. Declercq and co-workers from the animal facility (UMR7622). This work was supported by the CNRS, UPMC, La Ligue R{\'e}gionale Ile-de-France (RS11/75-38, RS12/75/95-6) and an ANR grant (ANR-12-BSV2-0005-01) to K. Wassmann. Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",
year = "2015",
month = apr,
day = "21",
doi = "10.1038/ncomms7946",
language = "English (US)",
volume = "6",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
}