TY - JOUR
T1 - Molecular karyotype analysis of Perkinsus atlanticus (Phylum Perkinsozoa) by pulsed field gel electrophoresis
AU - Leonor Teles-Grilo, M.
AU - Duarte, Sérgio M.
AU - Tato-Costa, Joana
AU - Gaspar-Maia, Alexandre
AU - Oliveira, Carla
AU - Rocha, António A.
AU - Marques, Américo
AU - Cordeiro-da-Silva, Anabela
AU - Azevedo, Carlos
N1 - Funding Information:
The authors thank Prof. Victor Costa and Dr. Frederico Silva from UP 3 -Protein Production and Purification Unit, Institute for Molecular and Cellular Biology (IBMC), University of Porto, for helping in densitometric analysis. This work was supported by the Foundation for Science and Technology (FCT/MCT) (Project no. POCTI/CTA/47583/2002).
PY - 2007/11/5
Y1 - 2007/11/5
N2 - Perkinsus atlanticus is a pathogenic protist that infects the clam Ruditapes decussatus. Although it was recently proposed that the genus Perkinsus belongs to a new phylum, Perkinsozoa, in the infra-kingdom Alveolata, there remain different opinions about whether this genus should form a phylum on its own and consequently divergent views about its taxonomic characterization. In this work, we have identified nine chromosomes by pulsed field gel electrophoresis (PFGE) combined with densitometry analysis. The obtained karyotype of Perkinsus atlanticus, like that of other early branches of the dinoflagellate lineage, displays a more conventional chromosome organization, different from that of most dinoflagellates.
AB - Perkinsus atlanticus is a pathogenic protist that infects the clam Ruditapes decussatus. Although it was recently proposed that the genus Perkinsus belongs to a new phylum, Perkinsozoa, in the infra-kingdom Alveolata, there remain different opinions about whether this genus should form a phylum on its own and consequently divergent views about its taxonomic characterization. In this work, we have identified nine chromosomes by pulsed field gel electrophoresis (PFGE) combined with densitometry analysis. The obtained karyotype of Perkinsus atlanticus, like that of other early branches of the dinoflagellate lineage, displays a more conventional chromosome organization, different from that of most dinoflagellates.
KW - Alveolata
KW - Molecular karyotype
KW - Perkinsozoa
KW - Perkinsus atlanticus
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U2 - 10.1016/j.ejop.2007.06.003
DO - 10.1016/j.ejop.2007.06.003
M3 - Article
C2 - 17822886
AN - SCOPUS:35148883833
SN - 0932-4739
VL - 43
SP - 315
EP - 318
JO - European Journal of Protistology
JF - European Journal of Protistology
IS - 4
ER -