Modeling, analysis and optimization of calibration uncertainty in clinical laboratories

Varun Ramamohan, James T. Abbott, George G. Klee, Yuehwern Yih

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Uncertainty in the calibration of a clinical laboratory measurement process has a significant effect on the uncertainty of the measurement result. We develop a mathematical model of the analytical stage of the measurement of serum triglyceride concentration in the clinical laboratory, and use the Monte Carlo method to estimate the net uncertainty associated with this model. We then use the model to study the effect of instrument calibration on the uncertainty of the laboratory measurement result. The effect of the correlation between the parameters of the linear calibration function on the measurement result is quantified using the model. In addition, the effect of the choice of calibrator concentration levels on the measurement result distribution is studied using the model, by studying the effect of the value or the position of the calibrator concentration, and the difference or the distance between calibrator concentrations, on the uncertainty of the measurement result.

Original languageEnglish (US)
Pages (from-to)175-185
Number of pages11
JournalMeasurement: Journal of the International Measurement Confederation
Issue number1
StatePublished - Apr 2014


  • Calibration correlation analysis
  • Clinical laboratory measurements
  • Optimal calibrator selection
  • Serum triglycerides

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering


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