Methods for cell sorting of young and senescent cells

João F. Passos, Thomas Von Zglinicki

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations


Cellular senescence, the ultimate and irreversible loss of replicative capacity of cells in primary culture, has been a popular model for studying the aging process. However, the replicative life span of human fibroblasts is heterogeneous even in clonal populations, with the fraction of senescent cells increasing at each population doubling, rather than all cells entering senescence simultaneously. Thus, the study of individual cells in a mass culture is of extreme importance to the understanding of replicative senescence. Cell sorting is a method that allows physical separation of cells with different characteristics when measured by flow cytometry. Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations.

Original languageEnglish (US)
Title of host publicationBiological Aging
Subtitle of host publicationMethods and Protocols
EditorsTrygve O. Tollefsbol
Number of pages12
StatePublished - Apr 5 2007

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • BrdU
  • Cell sorting
  • H2A.X
  • Heterogeneity
  • Ki67
  • MMP
  • ROS
  • Replicative senescence
  • γ

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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