TY - JOUR
T1 - Method of morphometric evaluation of spinal and autonomic ganglia
AU - Offord, Kenneth
AU - Ohta, Michiya
AU - Oenning, Roger F.
AU - Dyck, Peter James
N1 - Funding Information:
To measure the ganglion volume and the number and sizes of nerve cells, the ganglion has to be removed in its entirety at autopsy. In these studies fixation has been according to the schedule outlined by Ohta, Offord and Dyck (1974). The ganglion is embedded in paraffin and serially sectioned in the transverse plane at a constant section thickness. A staining procedure that produces intensely-stained nucleoli and enough staining of other cellular elements to facilitate measurement of the diameter of nerve cells is required. For This investigation was supported in part by Research Grants NS-5811 and NS-7541 and Research Contract 72-2200 from the National Institutes of Health, Public Health Service, and by grants from the Gallmeyer Fund, Miller Fund, Upton Fund, and Heller Fund.
PY - 1974/5
Y1 - 1974/5
N2 - We have demonstrated that, in determining the number of cytons (cell bodies) in a ganglion, it is important to consider the range of sizes of cytons and their associated nucleoli in correcting for the split-cell counting error. We have established the mathematical relationship between nucleolus diameter and cytons diameter microscopically in serial sections. An improved method, utilizing semi-automatic techniques and programmed calculation and plotting, is presented for estimating the volume of the ganglion, the number of cytons of neurons per ganglion, and the corrected frequency distribution of diameters of cytons of neurons.
AB - We have demonstrated that, in determining the number of cytons (cell bodies) in a ganglion, it is important to consider the range of sizes of cytons and their associated nucleoli in correcting for the split-cell counting error. We have established the mathematical relationship between nucleolus diameter and cytons diameter microscopically in serial sections. An improved method, utilizing semi-automatic techniques and programmed calculation and plotting, is presented for estimating the volume of the ganglion, the number of cytons of neurons per ganglion, and the corrected frequency distribution of diameters of cytons of neurons.
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U2 - 10.1016/0022-510X(74)90054-9
DO - 10.1016/0022-510X(74)90054-9
M3 - Article
C2 - 4598264
AN - SCOPUS:0016239224
SN - 0022-510X
VL - 22
SP - 65
EP - 71
JO - Journal of the neurological sciences
JF - Journal of the neurological sciences
IS - 1
ER -