TY - JOUR
T1 - Mechanisms underlying myosin heavy chain expression during development of the rat diaphragm muscle
AU - Geiger, Paige C.
AU - Bailey, Jeffrey P.
AU - Mantilla, Carlos B.
AU - Zhan, Wen Zhi
AU - Sieck, Gary C.
PY - 2006/12
Y1 - 2006/12
N2 - During early post-natal development in rat diaphragm muscle (Dia m), significant transitions in myosin heavy chain (MHC) isoform expression occur that are associated with fiber growth and increased MHC protein. At present, there is no direct information regarding the transcriptional regulation of MHC isoform expression during postnatal Dia m development. We hypothesized postnatal changes in MHC isoform mRNA expression are followed by concomitant changes in MHC protein expression. The Diam was removed at postnatal days 0, 14, 28, and 84 (adult). MHC mRNA expression was determined by real-time RT-PCR. MHC protein expression was determined by SDS-PAGE. There was a significant effect of postnatal age on MHC isoform mRNA and protein expression. At birth, the MHCNeo isoform accounted for 28% of MHC mRNA and 54% of total MHC protein. By postnatal day 14, MHCNeo mRNA and protein increased significantly, and both decreased significantly by day 28, consistent with transcriptional control of the expression of this developmental isoform. By postnatal day 28, there were minimal changes in mRNA expression for MHCSlow and MHC2X, yet protein expression increased significantly. MHC2A mRNA and protein expression did not change during this time. Thus changes in MHC protein expression did not follow (or parallel) changes in MHC mRNA for the adult MHC isoforms. The present findings indicate that changes in MHC expression in the developing rat Diam are not driven solely by changes in mRNA expression. Knowledge of isoform-specific MHC mRNA expression only yields predictive information on MHC protein expression for the MHCNeo isoform.
AB - During early post-natal development in rat diaphragm muscle (Dia m), significant transitions in myosin heavy chain (MHC) isoform expression occur that are associated with fiber growth and increased MHC protein. At present, there is no direct information regarding the transcriptional regulation of MHC isoform expression during postnatal Dia m development. We hypothesized postnatal changes in MHC isoform mRNA expression are followed by concomitant changes in MHC protein expression. The Diam was removed at postnatal days 0, 14, 28, and 84 (adult). MHC mRNA expression was determined by real-time RT-PCR. MHC protein expression was determined by SDS-PAGE. There was a significant effect of postnatal age on MHC isoform mRNA and protein expression. At birth, the MHCNeo isoform accounted for 28% of MHC mRNA and 54% of total MHC protein. By postnatal day 14, MHCNeo mRNA and protein increased significantly, and both decreased significantly by day 28, consistent with transcriptional control of the expression of this developmental isoform. By postnatal day 28, there were minimal changes in mRNA expression for MHCSlow and MHC2X, yet protein expression increased significantly. MHC2A mRNA and protein expression did not change during this time. Thus changes in MHC protein expression did not follow (or parallel) changes in MHC mRNA for the adult MHC isoforms. The present findings indicate that changes in MHC expression in the developing rat Diam are not driven solely by changes in mRNA expression. Knowledge of isoform-specific MHC mRNA expression only yields predictive information on MHC protein expression for the MHCNeo isoform.
KW - Electrophoresis
KW - Muscle plasticity
KW - Myosin heavy chain gene regulation
KW - Real-time reverse transcriptase-polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=33845439850&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845439850&partnerID=8YFLogxK
U2 - 10.1152/japplphysiol.00221.2006
DO - 10.1152/japplphysiol.00221.2006
M3 - Article
C2 - 16873604
AN - SCOPUS:33845439850
SN - 8750-7587
VL - 101
SP - 1546
EP - 1555
JO - Journal of applied physiology
JF - Journal of applied physiology
IS - 6
ER -