Mechanism of relaxations to C-type natriuretic peptide in veins

Michelle Banks, Chi Ming Wei, Cheol H. Kim, John C. Burnett, Virginia M. Miller

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


C-type natriuretic peptide (CNP) is an endothelium-derived peptide that shares structural homology with atrial natriuretic peptide (ANP). CNP causes greater endothelium-independent relaxations in veins compared with arteries. Relaxations to CNP in porcine coronary arteries are mediated by hyperpolarization of the smooth muscle membrane. Experiments were designed to investigate the mechanism(s) by which CNP causes relaxation in canine femoral veins. Rings of canine femoral veins without endothelium were suspended for measurement of isometric force in organ chambers. Concentration-response curves to CNP were obtained in veins contracted with either endothelin-1 (10-8 M), KCl (40 mM), phenylephrine (10-6 M) or prostaglandin F(2α) (2 x 10-6 M) in the absence and presence of BQ-123 (10-6 M), N(G)- monomenthyl-L-arginine (L-NMMA; 10-4 M), HS-142-1 (10-5 M), methylene blue (10-5 M), or potassium channel blockers, tetraethylammonium chloride (TEA; 10-3 M), charybdotoxin (10-7 M), glibenclamide (10-7 M), or apamin (10-7 M). Relaxations to CNP were significantly attenuated when the tissue was contracted with KCl and endothelin-1. During contraction to either phenylephrine or prostaglandin F(2α), relaxations to CNP were inhibited by HS-142-1, methylene blue, TEA, and charybdotoxin, but not by L-NMMA, glibenclamide, or apamin. In separate experiments, guanosine 3',5'-cyclic monophosphate increased twofold within 10-60 s after the addition of CNP (10-8 M). These data suggest that CNP mediates relaxation of canine femoral veins through activation of large-conduction, calcium-activated potassium channels and activation of particulate and soluble guanylate cyclase.

Original languageEnglish (US)
Pages (from-to)H1907-H1911
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number5 40-5
StatePublished - Nov 1996


  • canine femoral veins
  • guanosine 3',5'-cyclic monophosphate
  • hyperpolarization

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


Dive into the research topics of 'Mechanism of relaxations to C-type natriuretic peptide in veins'. Together they form a unique fingerprint.

Cite this