Measurement of plasma acetate kinetics using high-performance liquid chromatography

M. Persson; B. Bleiberg; D. Kiss; J. Miles

doi:10.1016/0003-2697(91)90520-4

Measurement of plasma acetate kinetics using high-performance liquid chromatography

M. Persson, B. Bleiberg, D. Kiss, J. Miles

Endocrinology, Diabetes, Metabolism, and Nutrition

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16 Scopus citations

Abstract

Previous studies suggest that plasma acetate may be an important fuel in man, accounting for ∼ 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of d-β-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [¹⁴C]acetate into red blood cells was negligible.

Original language	English (US)
Pages (from-to)	149-153
Number of pages	5
Journal	Analytical Biochemistry
Volume	198
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(91)90520-4
State	Published - Oct 1991

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Measurement of plasma acetate kinetics using high-performance liquid chromatography",

abstract = "Previous studies suggest that plasma acetate may be an important fuel in man, accounting for ∼ 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of d-β-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [14C]acetate into red blood cells was negligible.",

author = "M. Persson and B. Bleiberg and D. Kiss and J. Miles",

note = "Funding Information: We thank P. Voelker for editorial assistance. This work ported in part by grants from the U.S. Public Health (DK38092) and the Mayo Foundation.",

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AU - Persson, M.

AU - Bleiberg, B.

AU - Kiss, D.

AU - Miles, J.

N1 - Funding Information: We thank P. Voelker for editorial assistance. This work ported in part by grants from the U.S. Public Health (DK38092) and the Mayo Foundation.

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N2 - Previous studies suggest that plasma acetate may be an important fuel in man, accounting for ∼ 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of d-β-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [14C]acetate into red blood cells was negligible.

AB - Previous studies suggest that plasma acetate may be an important fuel in man, accounting for ∼ 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of d-β-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [14C]acetate into red blood cells was negligible.

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Measurement of plasma acetate kinetics using high-performance liquid chromatography

Abstract

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