TY - JOUR
T1 - Measles virus editing provides an additional cysteine-rich protein
AU - Cattaneo, Roberto
AU - Kaelin, Karin
AU - Baczko, Knut
AU - Billeter, Martin A.
N1 - Funding Information:
We are greatly indebted to Reay Paterson, Robert Lamb, and Sheila Thomas for transmission of the preprint describing their results with SV5 prior to publication, and to Dan Kolakofsky for his vivid interest in the described phenomena and his support in the exchange and dissemination of these data. We thank Anita Schmid and Daniel Eschle for plasmids and advice, Daniel Schijmperli for his careful review of the manuscript, Deborah Maguire for linguistic assistance, Fritz Ochsen-bein for the photographs, and Bert Rima, Steve Udem, and Charles Weissmann for discussions. This work was presented in the meeting on Negative Strand Viruses held in Dinard, France, September 18-23, 1988; it was supported by grant 3.141.085 of the Schweizerische Na-tionalfonds and by the Kanton Ziirich. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1989/3/10
Y1 - 1989/3/10
N2 - The measles virus (MV) phosphoprotein (P) gene encodes two known proteins, P (Mr ≈ 70,000), involved in viral transcription, and, in a different reading frame, C (Mr ≈ 20,000). By a combination of cDNA cloning, cDNA and RNA sequencing, and in vitro translation, we demonstrate here that the MV P gene also expresses a third product (Mr ≈ 46,000) containing the amino-terminal region of P but a different, cysteinerich carboxy-terminal motif. This third protein is translated from mRNAs in which one G residue has been inserted after three genomically encoded Gs, a modification found in about 50% of the P mRNAs. A smaller fraction of transcripts contain several additional G residues.
AB - The measles virus (MV) phosphoprotein (P) gene encodes two known proteins, P (Mr ≈ 70,000), involved in viral transcription, and, in a different reading frame, C (Mr ≈ 20,000). By a combination of cDNA cloning, cDNA and RNA sequencing, and in vitro translation, we demonstrate here that the MV P gene also expresses a third product (Mr ≈ 46,000) containing the amino-terminal region of P but a different, cysteinerich carboxy-terminal motif. This third protein is translated from mRNAs in which one G residue has been inserted after three genomically encoded Gs, a modification found in about 50% of the P mRNAs. A smaller fraction of transcripts contain several additional G residues.
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U2 - 10.1016/0092-8674(89)90679-X
DO - 10.1016/0092-8674(89)90679-X
M3 - Article
C2 - 2924348
AN - SCOPUS:0024519674
SN - 0092-8674
VL - 56
SP - 759
EP - 764
JO - Cell
JF - Cell
IS - 5
ER -