TY - JOUR
T1 - Magnetic resonance characterization of tissue engineered cartilage via changes in relaxation times, diffusion coefficient, and shear modulus
AU - Yin, Ziying
N1 - Publisher Copyright:
© 2014 by Begell House, Inc.
PY - 2014
Y1 - 2014
N2 - The primary goal of this paper is to describe a combined MR relaxation (T2 and T1ρ), diffusion (apparent diffusion coefficient [ADC]), and elastography (shear stiffness) method of fully characterizing the development of tissue-engineered cartilage in terms of the changes in its composition, structure, and mechanical properties during tissue growth. Then, we may better use MR-based methodologies to noninvasively monitor and optimize the cartilage tissue engineering process without sacrificing the constructs. This process begins by demonstrating the potential capability of T2, T1ρ, ADC, and shear stiffness in characterizing a scaffold-free engineered cartilage. The results show that, in addition to the conventional T2 and ADC, T1ρ and MRE can be used as potential biomarkers to assess the specific changes in proteoglycan content and mechanical properties of engineered cartilage during culture. Moreover, to increase the efficiency of MR characterization, two new methodologies for simultaneous acquisition of diffusion and MRE (dMRE), and T1ρ and MRE (T1ρ-MRE) are introduced that allow the simultaneous characterization of both biochemical and mechanical properties of engineered cartilage tissue. The feasibilities of dMRE and T1ρ-MRE approaches are validated on tissue-mimicking phantoms. The results show good correspondence between simultaneous acquisitions and conventional separate acquisition methods.
AB - The primary goal of this paper is to describe a combined MR relaxation (T2 and T1ρ), diffusion (apparent diffusion coefficient [ADC]), and elastography (shear stiffness) method of fully characterizing the development of tissue-engineered cartilage in terms of the changes in its composition, structure, and mechanical properties during tissue growth. Then, we may better use MR-based methodologies to noninvasively monitor and optimize the cartilage tissue engineering process without sacrificing the constructs. This process begins by demonstrating the potential capability of T2, T1ρ, ADC, and shear stiffness in characterizing a scaffold-free engineered cartilage. The results show that, in addition to the conventional T2 and ADC, T1ρ and MRE can be used as potential biomarkers to assess the specific changes in proteoglycan content and mechanical properties of engineered cartilage during culture. Moreover, to increase the efficiency of MR characterization, two new methodologies for simultaneous acquisition of diffusion and MRE (dMRE), and T1ρ and MRE (T1ρ-MRE) are introduced that allow the simultaneous characterization of both biochemical and mechanical properties of engineered cartilage tissue. The feasibilities of dMRE and T1ρ-MRE approaches are validated on tissue-mimicking phantoms. The results show good correspondence between simultaneous acquisitions and conventional separate acquisition methods.
KW - Diffusion
KW - Elastography
KW - Imaging
KW - Magnetic resonance
KW - Relaxation
KW - Simultaneous acquisition
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U2 - 10.1615/CritRevBiomedEng.2014011843
DO - 10.1615/CritRevBiomedEng.2014011843
M3 - Article
C2 - 25403876
AN - SCOPUS:84921842323
SN - 0278-940X
VL - 42
SP - 137
EP - 191
JO - Critical Reviews in Biomedical Engineering
JF - Critical Reviews in Biomedical Engineering
IS - 2
ER -