Macrophage MicroRNA-155 promotes cardiac hypertrophy and failure

Stephane Heymans, Maarten F. Corsten, Wouter Verhesen, Paolo Carai, Rick E.W. Van Leeuwen, Kevin Custers, Tim Peters, Mark Hazebroek, Lauran Stöger, Erwin Wijnands, Ben J. Janssen, Esther E. Creemers, Yigal M. Pinto, Dirk Grimm, Nina Schürmann, Elena Vigorito, Thomas Thum, Frank Stassen, Xiaoke Yin, Manuel MayrLeon J. De Windt, Esther Lutgens, Kristiaan Wouters, Menno P.J. De Winther, Serena Zacchigna, Mauro Giacca, Marc Van Bilsen, Anna Pia Papageorgiou, Blanche Schroen

Research output: Contribution to journalArticlepeer-review


BACKGROUND - : Cardiac hypertrophy and subsequent heart failure triggered by chronic hypertension represent major challenges for cardiovascular research. Beyond neurohormonal and myocyte signaling pathways, growing evidence suggests inflammatory signaling pathways as therapeutically targetable contributors to this process. We recently reported that microRNA-155 is a key mediator of cardiac inflammation and injury in infectious myocarditis. Here, we investigated the impact of microRNA-155 manipulation in hypertensive heart disease. METHODS AND RESULTS - : Genetic loss or pharmacological inhibition of the leukocyte-expressed microRNA-155 in mice markedly reduced cardiac inflammation, hypertrophy, and dysfunction on pressure overload. These alterations were macrophage dependent because in vivo cardiomyocyte-specific microRNA-155 manipulation did not affect cardiac hypertrophy or dysfunction, whereas bone marrow transplantation from wild-type mice into microRNA-155 knockout animals rescued the hypertrophic response of the cardiomyocytes and vice versa. In vitro, media from microRNA-155 knockout macrophages blocked the hypertrophic growth of stimulated cardiomyocytes, confirming that macrophages influence myocyte growth in a microRNA-155-dependent paracrine manner. These effects were at least partly mediated by the direct microRNA-155 target suppressor of cytokine signaling 1 (Socs1) because Socs1 knockdown in microRNA-155 knockout macrophages largely restored their hypertrophy-stimulating potency. CONCLUSIONS - : Our findings reveal that microRNA-155 expression in macrophages promotes cardiac inflammation, hypertrophy, and failure in response to pressure overload. These data support the causative significance of inflammatory signaling in hypertrophic heart disease and demonstrate the feasibility of therapeutic microRNA targeting of inflammation in heart failure.

Original languageEnglish (US)
Pages (from-to)1420-1432
Number of pages13
Issue number13
StatePublished - Sep 24 2013


  • Heart failure
  • hypertrophy
  • inflammation
  • microRNAs

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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