@article{8e4a36d6cf434540b843b217ed0b294b,
title = "Large Differences in Small RNA Composition Between Human Biofluids",
abstract = "Extracellular microRNAs (miRNAs) and other small RNAs are implicated in cellular communication and may be useful as disease biomarkers. We systematically compared small RNAs in 12 human biofluid types using RNA sequencing (RNA-seq). miRNAs and tRNA-derived RNAs (tDRs) accounted for the majority of mapped reads in all biofluids, but the ratio of miRNA to tDR reads varied from 72 in plasma to 0.004 in bile. miRNA levels were highly correlated across all biofluids, but levels of some miRNAs differed markedly between biofluids. tDR populations differed extensively between biofluids. Y RNA fragments were seen in all biofluids and accounted for >10% of reads in blood plasma, serum, and cerebrospinal fluid (CSF). Reads mapping exclusively to Piwi-interacting RNAs (piRNAs) were very rare, except in seminal plasma. These results demonstrate extensive differences in small RNAs between human biofluids and provide a useful resource for investigating extracellular RNA biology and developing biomarkers. Using a standardized sequencing-based approach, Godoy et al. characterize small RNAs in 12 normal human biofluids. They find that each biofluid contains an extensive collection of small RNAs that belong to multiple biotypes. The relative abundance of these RNAs varies widely between biofluids.",
keywords = "Y RNA, biofluids, extracellular RNA, miRNA, tRNA",
author = "Godoy, {Paula M.} and Bhakta, {Nirav R.} and Barczak, {Andrea J.} and Hakan Cakmak and Susan Fisher and MacKenzie, {Tippi C.} and Tushar Patel and Price, {Richard W.} and Smith, {James F.} and Woodruff, {Prescott G.} and Erle, {David J.}",
note = "Funding Information: This publication is part of the NIH ERCC paper package and was supported by the NIH Common Fund's exRNA Communication Program. We thank the individuals who participated in the study by providing samples; Christine P. Nguyen and Bobby Antalek for assistance with collection and annotation of the BAL fluid, adult blood plasma, serum, and urine samples; Serena Spudich for access to CSF samples collected in studies that she directed; Michael Li and Suresh Garudadri for performing qPCR experiments; and William Thistlethwaite (Baylor College of Medicine) for help with depositing data. This work was supported by the NIH Extracellular RNA Communication Program (grant U01HL126493 to P.G.W. and D.J.E. and grants UH2TR000884 and UH3TR000884 to T.P.). Funding Information: This publication is part of the NIH ERCC paper package and was supported by the NIH Common Fund{\textquoteright}s exRNA Communication Program. We thank the individuals who participated in the study by providing samples; Christine P. Nguyen and Bobby Antalek for assistance with collection and annotation of the BAL fluid, adult blood plasma, serum, and urine samples; Serena Spudich for access to CSF samples collected in studies that she directed; Michael Li and Suresh Garudadri for performing qPCR experiments; and William Thistlethwaite (Baylor College of Medicine) for help with depositing data. This work was supported by the NIH Extracellular RNA Communication Program (grant U01HL126493 to P.G.W. and D.J.E. and grants UH2TR000884 and UH3TR000884 to T.P.). Publisher Copyright: {\textcopyright} 2018 The Authors",
year = "2018",
month = oct,
day = "30",
doi = "10.1016/j.celrep.2018.10.014",
language = "English (US)",
volume = "25",
pages = "1346--1358",
journal = "Cell reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}