TY - JOUR
T1 - Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging
AU - Li, Cai
AU - Shklover, Jeny
AU - Parvizi, Mojtaba
AU - Sherlock, Benjamin E.
AU - Alfonso Garcia, Alba
AU - Haudenschild, Anne K.
AU - Griffiths, Leigh G.
AU - Marcu, Laura
N1 - Publisher Copyright:
© 2018, Biomedical Engineering Society.
PY - 2018/11/15
Y1 - 2018/11/15
N2 - The extracellular matrix architecture of bovine pericardium (BP) has distinct biochemical and biomechanical properties that make it a useful biomaterial in the field of regenerative medicine. Collagen represents the dominant structural protein of BP and is therefore intimately associated with the properties of this biomaterial. Enzymatic degradation of collagen molecules is critical for extracellular matrix turnover, remodeling and ultimately tissue regeneration. We present a quantitative, label-free and non-destructive method for monitoring changes in biochemical and biomechanical properties of BP during tissue degradation, based on multi-spectral fluorescence lifetime imaging (ms-FLIm). Strong correlations of fluorescence intensity ratio and average fluorescence lifetime were identified with collagen content, Young’s Modulus and Ultimate tensile strength during collagenase degradation, indicating the potential of optically monitoring collagen degradation using ms-FLIm. The obtained results demonstrate the value of ms-FLIm to assess the quality of biomaterials in situ for applications in regenerative medicine.
AB - The extracellular matrix architecture of bovine pericardium (BP) has distinct biochemical and biomechanical properties that make it a useful biomaterial in the field of regenerative medicine. Collagen represents the dominant structural protein of BP and is therefore intimately associated with the properties of this biomaterial. Enzymatic degradation of collagen molecules is critical for extracellular matrix turnover, remodeling and ultimately tissue regeneration. We present a quantitative, label-free and non-destructive method for monitoring changes in biochemical and biomechanical properties of BP during tissue degradation, based on multi-spectral fluorescence lifetime imaging (ms-FLIm). Strong correlations of fluorescence intensity ratio and average fluorescence lifetime were identified with collagen content, Young’s Modulus and Ultimate tensile strength during collagenase degradation, indicating the potential of optically monitoring collagen degradation using ms-FLIm. The obtained results demonstrate the value of ms-FLIm to assess the quality of biomaterials in situ for applications in regenerative medicine.
KW - Collagenase degradation
KW - Fluorescence lifetime imaging
KW - Non-destructive monitoring
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U2 - 10.1007/s10439-018-2087-6
DO - 10.1007/s10439-018-2087-6
M3 - Article
C2 - 30003502
AN - SCOPUS:85049788406
SN - 0090-6964
VL - 46
SP - 1870
EP - 1881
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 11
ER -