Junctophilin-2 is necessary for T-tubule maturation during mouse heart development

Julia O. Reynolds, David Y. Chiang, Wei Wang, David L. Beavers, Sayali S. Dixit, Darlene G. Skapura, Andrew P. Landstrom, Long Sheng Song, Michael J. Ackerman, Xander H.T. Wehrens

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


Aims: Transverse tubules (TTs) provide the basic subcellular structuresthat facilitate excitation-contraction (EC) coupling, the essential process that underlies normal cardiac contractility. Previous studies have shown that TTs develop within thefirst few weeks of life in mammals but the molecular determinants of this development have remained elusive. This study aims to elucidate the role of junctophilin-2 (JPH2), a junctional membrane complex protein, in the maturation of TTs in car-diomyocytes. Methods and results: Using a novel cardiac-specific short-hairpin-RNA-mediated JPH2 knockdown mouse model (Mus musculus; αMHC-shJPH2), weassessed the effects of the loss of JPH2 on the maturation of the ventricular TT structure. Between embryonic day (E) 10.5 and postnatal day (P) 10, JPH2 mRNA and protein levels were reduced by >70% in αMHC-shJPH2 mice. At P8 and P10, knockdown of JPH2 significantly inhibited the maturation of TTs, while expression levels of other genes implicated in TT development remained mostly unchanged. At the same time, intracellular Ca2+ handling was disrupted in ventricular myocytes from αMHC-shJPH2 mice, which developed heart failure by P10 marked by reduced ejection fraction, ventricular dilation, and premature death. In contrast, JPH2 transgenic mice exhibited accelerated TT maturation by P8. Conclusion: Our findings suggest that JPH2 is necessary for TT maturation during postnatal cardiac developmentin mice. In particular, JPH2 may be critical in anchoring the invaginating sarcolemma to the sarcoplasmic reticulum, thereby enabling the maturation of the TT network.

Original languageEnglish (US)
Pages (from-to)44-53
Number of pages10
JournalCardiovascular research
Issue number1
StatePublished - Oct 1 2013


  • Developmental biology
  • E-C coupling
  • Junctophilin-2
  • Transverse tubules

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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