Isolation and characterization of two new functional subtypes of HLA‐B35

Graciela Theiler, Marcelo Pando, José M. Delfino, Masafumi Takiguchi, M. Leonardo Satz

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Human minor histocompatibility antigen‐specific, HLA‐B*3501‐restricted cytotoxic T‐cell clones were assayed on a panel of 25 different target cells previously typed by serology as HLA‐B35. Cells from 6 donors were not killed and cells from 2 of these were further studied by molecular cloning to characterize their HLA class I alleles. Two new HLA‐B35 subtypes were identified. The sequence of one of them differs from B*3501 by one nucleotide change at codon 156, replacing a Leu for an Arg. The sequence of the other new subtype also shows a single nucleotide change compared to B*3501, with Gly Val substitution at residue 16. With these new variants, the allelic complexity of HLA‐B35 extends now to eight subtypes. 1993 Blackwell Munksgaard

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalTissue Antigens
Issue number3
StatePublished - Mar 1993


  • HLA‐B35 subtypes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics


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