TY - JOUR
T1 - Intragenic recombination in an E(β) gene for a murine Ia antigen
AU - Plunkett, M. L.
AU - Coligan, J. E.
AU - David, C. S.
AU - Freed, J. H.
PY - 1982
Y1 - 1982
N2 - The recombinant strain D2.GD was originally typed as I-A(d) by serological methods. Indeed, the A(α) and A(β) chains of the I-A antigens appear to exhibit normal behavior by the criteria of serology and two dimensional gel analysis. However, the E(β) chain encoded by the I-A subregion of this strain, one of the two components of the plasma membrane located I-E antigens produced in (D2.GD x A.TFR5)F1 animals, has been demonstrated to be the product of an intragenic recombinational event between E(β) genes from the d and b haplotypes. Sequence analysis suggests that the amino-terminal portion of the E(β)(g2) chain is derived from the d haplotype and, therefore, that the coding strand for this gene is oriented centromeric → telomeric (5' to 3' direction). Finally, these data combined with the data of Rose and Cullen allow the ordering of the genes within the I-A subregion as (H-2K), A(α), A(β), E(β) ... (H-2D).
AB - The recombinant strain D2.GD was originally typed as I-A(d) by serological methods. Indeed, the A(α) and A(β) chains of the I-A antigens appear to exhibit normal behavior by the criteria of serology and two dimensional gel analysis. However, the E(β) chain encoded by the I-A subregion of this strain, one of the two components of the plasma membrane located I-E antigens produced in (D2.GD x A.TFR5)F1 animals, has been demonstrated to be the product of an intragenic recombinational event between E(β) genes from the d and b haplotypes. Sequence analysis suggests that the amino-terminal portion of the E(β)(g2) chain is derived from the d haplotype and, therefore, that the coding strand for this gene is oriented centromeric → telomeric (5' to 3' direction). Finally, these data combined with the data of Rose and Cullen allow the ordering of the genes within the I-A subregion as (H-2K), A(α), A(β), E(β) ... (H-2D).
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U2 - 10.1084/jem.155.3.937
DO - 10.1084/jem.155.3.937
M3 - Article
C2 - 6950022
AN - SCOPUS:0020045544
SN - 0022-1007
VL - 155
SP - 937
EP - 942
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 3
ER -