Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur

Fangjun Liu, James W. Wells, Ryan M. Porter, Vaida Glatt, Zhenxin Shen, Martina Schinhan, Alan Ivkovic, Mark S. Vrahas, Christopher H. Evans, Elisabeth Ferreira

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Orthopedic surgeons sometimes combine recombinant, human BMP-2 with autograft bone when dealing with problematic osseous fractures. Although some case reports indicate success with this off-label strategy, there have been no randomized controlled trials. Moreover, a literature search revealed only one pre-clinical study and this was in a cranial defect model. The present project examined the consequences of combining BMP-2 with particles of living bone in a rat femoral defect model. Human bone particles were recovered with a reamer-irrigator-aspirator (RIA). To allow acceptance of the xenograft as surrogate autograft, rats were administered an immunosuppressive cocktail that does not interfere with bone healing. Implantation of 200 µg living bone particles generated a small amount of new bone and defects did not heal. Graded amounts of BMP-2 that alone provoked no healing (1.1 µg), borderline healing (5.5 µg), or full healing (11 µg) were added to this amount of bone particles. Addition of BMP-2 (1.1 µg) increased osteogenesis, and produced bridging in 2 of 7 defects. The combination of BMP-2 (5.5 µg) and bone particles made healing more reliable and advanced the maturation of the regenerate. Bone formation with BMP-2 (11 µg) and bone particles showed improved maturation. Thus, the combination of autograft and BMP-2 may be helpful clinically under conditions where the healing response is suboptimal.

Original languageEnglish (US)
Pages (from-to)2137-2145
Number of pages9
JournalJournal of Orthopaedic Research
Issue number12
StatePublished - Dec 1 2016


  • RIA
  • autograft
  • immunosuppression
  • xenograft

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine


Dive into the research topics of 'Interaction between living bone particles and rhBMP-2 in large segmental defect healing in the rat femur'. Together they form a unique fingerprint.

Cite this