Insulin-modulated interleukin-2 production by murine splenocytes and a T-cell hybridoma

K. Pavelic; R. J. Bernacki; S. Vuk-Pavlovic

doi:10.1677/joe.0.1140089

Insulin-modulated interleukin-2 production by murine splenocytes and a T-cell hybridoma

K. Pavelic, R. J. Bernacki, S. Vuk-Pavlovic

Hematology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Murine interleukin-2 (IL-2)-producing AOFS 21 T-cell hybridoma cells and normal murine splenocytes were stimulated in serum-free media by 16 potential mitogens/growth factors. Only insulin, concanavalin A (Con A), peptidoglycan monomer and a tumour-derived insulinoid stimulated [³H]thymidine incorporation by AOFS 21 cells and splenocytes. Supernatants of these stimulated cultures were tested for IL-2 activity which generally followed the pattern of growth stimulation. Both the mitogenicity and stimulation of IL-2 secretion by insulin were second only to the effects of Con A.

Original language	English (US)
Pages (from-to)	89-94
Number of pages	6
Journal	Journal of Endocrinology
Volume	114
Issue number	1
DOIs	https://doi.org/10.1677/joe.0.1140089
State	Published - 1987

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

Access to Document

10.1677/joe.0.1140089

Cite this

@article{aef4f0d6a8054d0ca6fa376a9d157b07,

title = "Insulin-modulated interleukin-2 production by murine splenocytes and a T-cell hybridoma",

abstract = "Murine interleukin-2 (IL-2)-producing AOFS 21 T-cell hybridoma cells and normal murine splenocytes were stimulated in serum-free media by 16 potential mitogens/growth factors. Only insulin, concanavalin A (Con A), peptidoglycan monomer and a tumour-derived insulinoid stimulated [3H]thymidine incorporation by AOFS 21 cells and splenocytes. Supernatants of these stimulated cultures were tested for IL-2 activity which generally followed the pattern of growth stimulation. Both the mitogenicity and stimulation of IL-2 secretion by insulin were second only to the effects of Con A.",

author = "K. Pavelic and Bernacki, {R. J.} and S. Vuk-Pavlovic",

year = "1987",

doi = "10.1677/joe.0.1140089",

language = "English (US)",

volume = "114",

pages = "89--94",

journal = "Journal of Endocrinology",

issn = "0022-0795",

publisher = "Society for Endocrinology",

number = "1",

}

TY - JOUR

T1 - Insulin-modulated interleukin-2 production by murine splenocytes and a T-cell hybridoma

AU - Pavelic, K.

AU - Bernacki, R. J.

AU - Vuk-Pavlovic, S.

PY - 1987

Y1 - 1987

N2 - Murine interleukin-2 (IL-2)-producing AOFS 21 T-cell hybridoma cells and normal murine splenocytes were stimulated in serum-free media by 16 potential mitogens/growth factors. Only insulin, concanavalin A (Con A), peptidoglycan monomer and a tumour-derived insulinoid stimulated [3H]thymidine incorporation by AOFS 21 cells and splenocytes. Supernatants of these stimulated cultures were tested for IL-2 activity which generally followed the pattern of growth stimulation. Both the mitogenicity and stimulation of IL-2 secretion by insulin were second only to the effects of Con A.

AB - Murine interleukin-2 (IL-2)-producing AOFS 21 T-cell hybridoma cells and normal murine splenocytes were stimulated in serum-free media by 16 potential mitogens/growth factors. Only insulin, concanavalin A (Con A), peptidoglycan monomer and a tumour-derived insulinoid stimulated [3H]thymidine incorporation by AOFS 21 cells and splenocytes. Supernatants of these stimulated cultures were tested for IL-2 activity which generally followed the pattern of growth stimulation. Both the mitogenicity and stimulation of IL-2 secretion by insulin were second only to the effects of Con A.

UR - http://www.scopus.com/inward/record.url?scp=0023213972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023213972&partnerID=8YFLogxK

U2 - 10.1677/joe.0.1140089

DO - 10.1677/joe.0.1140089

M3 - Article

C2 - 3309115

AN - SCOPUS:0023213972

SN - 0022-0795

VL - 114

SP - 89

EP - 94

JO - Journal of Endocrinology

JF - Journal of Endocrinology

IS - 1

ER -

Insulin-modulated interleukin-2 production by murine splenocytes and a T-cell hybridoma

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this