Insulin-modulated interleukin-2 production by murine splenocytes and a T-cell hybridoma

K. Pavelic, R. J. Bernacki, S. Vuk-Pavlovic

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Murine interleukin-2 (IL-2)-producing AOFS 21 T-cell hybridoma cells and normal murine splenocytes were stimulated in serum-free media by 16 potential mitogens/growth factors. Only insulin, concanavalin A (Con A), peptidoglycan monomer and a tumour-derived insulinoid stimulated [3H]thymidine incorporation by AOFS 21 cells and splenocytes. Supernatants of these stimulated cultures were tested for IL-2 activity which generally followed the pattern of growth stimulation. Both the mitogenicity and stimulation of IL-2 secretion by insulin were second only to the effects of Con A.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalJournal of Endocrinology
Issue number1
StatePublished - 1987

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology


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