@article{e90ccec616554736b7ba3c15d4dfe7fa,
title = "Inhibition of the sonic hedgehog pathway by cyplopamine reduces the CD133+/CD15+ cell compartment and the in vitro tumorigenic capability of neuroblastoma cells",
abstract = "Sonic hedgehog (Hh) developmental pathway deregulation has been proven to play an essential role in several malignancies as neuroblastoma. We found that Hh signaling is active in neuroblastoma, as most pathway components, including GLI1, were expressed in cell lines and tumor samples. Furthermore, SHH ligand expression was found in cell lines and tumors, and GLI1 up-regulation was achieved in response to SHH treatment, suggesting an autocrine mechanism of aberrant activation. A decrease of proliferation and tumorigenic potential, as well as increased apoptosis and a dramatic decrease in the percentage of CD15+ cell population were produced upon Hh inhibition by cyclopamine.",
keywords = "CD133, CD15, Cyclopamine, Neuroblastoma, Sonic hedgehog",
author = "Paula Schiapparelli and Shahi, {Mehdi H.} and M{\'o}nica Enguita-Germ{\'a}n and Johnsen, {John Inge} and Per Kogner and Paula L{\'a}zcoz and Castresana, {Javier S.}",
note = "Funding Information: Authors are grateful to Laura Stokes for help with editing the manuscript. We would like to thank Izaskun Gabari from CIMA, University of Navarra, for performing flow cytometry analyses of CD133. P. Schiapparelli and M. Enguita-Germ{\'a}n would like to thank the Asociaci{\'o}n de Amigos de la Universidad de Navarra and the Departamento de Educaci{\'o}n del Gobierno de Navarra for the fellowships received. J.S. Castresana thank the Asociaci{\'o}n Espa{\~n}ola de Pediatr{\'i}a for the VIII Premio Nutrib{\'e}n de Investigaci{\'o}n Pedi{\'a}trica, Madrid. This study was supported in part by grants from the Departmento de Salud del Gobierno de Navarra (9/07), Caja Navarra, Fundaci{\'o}n Universitaria de Navarra, Pamplona, as well as Fondo de Investigaci{\'o}n Sanitaria (PI081849), Madrid. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",
year = "2011",
month = nov,
day = "28",
doi = "10.1016/j.canlet.2011.07.005",
language = "English (US)",
volume = "310",
pages = "222--231",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2",
}