TY - JOUR
T1 - Inflammatory Cells in Nephrectomy Tissue from Patients without and with a History of Urinary Stone Disease
AU - Dejban, Pegah
AU - Wilson, Elena M.
AU - Jayachandran, Muthuvel
AU - Herrera Hernandez, Loren P.
AU - Haskic, Zejfa
AU - Wellik, Linda E.
AU - Sinha, Sutapa
AU - Rule, Andrew D.
AU - Denic, Aleksandar
AU - Koo, Kevin
AU - Potretzke, Aaron M.
AU - Lieske, John C.
N1 - Publisher Copyright:
© 2022 by the American Society of Nephrology.
PY - 2022/3
Y1 - 2022/3
N2 - Background and objectives Urinary stone disease has been associated with inflammation, but the specific cell interactions that mediate events remain poorly defined. This study compared calcification and inflammatory cell patterns in kidney tissue from radical nephrectomy specimens of patients without and with a history of urinary stone disease. Design, setting, participants, & measurements Nontumor parenchyma of biobanked radical nephrectomy specimens from age-and sex-matched stone formers (n544) and nonstone formers (n582) were compared. Calcification was detected by Yasue staining and inflammatory cell populations by immunohistochemistry for CD68 (proinflammatory M1 macrophages), CD163 and CD206 (anti-inflammatory M2 macrophages), CD3 (T lymphocytes), and tryptase (mast cells). Calcifications and inflammatory cells were quantified in cortex and medulla using Image-Pro analysis software. Results Calcification in the medulla of stone formers was higher than in nonstone formers (P,0.001). M1 macrophages in the cortex and medulla of stone formers were greater than in nonstone formers (P,0.001), and greater in stone former medulla than stone former cortex (P50.02). There were no differences in age, sex, body mass index, tumor characteristics (size, stage, or thrombus), vascular disease status, or eGFR between the groups. M2 macrophages, T lymphocytes, and mast cells did not differ by stone former status. There was a correlation between M1 macrophages and calcification in the medulla of stone formers (rho50.48; P50.001) and between M2 macrophages and calcification in the medulla of nonstone formers (rho50.35; P50.001). T lymphocytes were correlated with calcification in the cortex of both nonstone formers (rho50.27; P50.01) and stone formers (rho50.42; P50.004), whereas mast cells and calcification were correlated only in the cortex of stone formers (rho50.35; P50.02). Conclusions Higher medullary calcification stimulated accumulation of proinflammatory rather than anti-inflammatory macrophages in stone formers.
AB - Background and objectives Urinary stone disease has been associated with inflammation, but the specific cell interactions that mediate events remain poorly defined. This study compared calcification and inflammatory cell patterns in kidney tissue from radical nephrectomy specimens of patients without and with a history of urinary stone disease. Design, setting, participants, & measurements Nontumor parenchyma of biobanked radical nephrectomy specimens from age-and sex-matched stone formers (n544) and nonstone formers (n582) were compared. Calcification was detected by Yasue staining and inflammatory cell populations by immunohistochemistry for CD68 (proinflammatory M1 macrophages), CD163 and CD206 (anti-inflammatory M2 macrophages), CD3 (T lymphocytes), and tryptase (mast cells). Calcifications and inflammatory cells were quantified in cortex and medulla using Image-Pro analysis software. Results Calcification in the medulla of stone formers was higher than in nonstone formers (P,0.001). M1 macrophages in the cortex and medulla of stone formers were greater than in nonstone formers (P,0.001), and greater in stone former medulla than stone former cortex (P50.02). There were no differences in age, sex, body mass index, tumor characteristics (size, stage, or thrombus), vascular disease status, or eGFR between the groups. M2 macrophages, T lymphocytes, and mast cells did not differ by stone former status. There was a correlation between M1 macrophages and calcification in the medulla of stone formers (rho50.48; P50.001) and between M2 macrophages and calcification in the medulla of nonstone formers (rho50.35; P50.001). T lymphocytes were correlated with calcification in the cortex of both nonstone formers (rho50.27; P50.01) and stone formers (rho50.42; P50.004), whereas mast cells and calcification were correlated only in the cortex of stone formers (rho50.35; P50.02). Conclusions Higher medullary calcification stimulated accumulation of proinflammatory rather than anti-inflammatory macrophages in stone formers.
KW - Calcification
KW - Inflammation
KW - Macrophage
KW - Medulla
KW - Nephrectomy
KW - Nephrolithiasis
KW - Urologic diseases
UR - http://www.scopus.com/inward/record.url?scp=85125966857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85125966857&partnerID=8YFLogxK
U2 - 10.2215/CJN.11730921
DO - 10.2215/CJN.11730921
M3 - Article
C2 - 35078782
AN - SCOPUS:85125966857
SN - 1555-9041
VL - 17
SP - 414
EP - 422
JO - Clinical Journal of the American Society of Nephrology
JF - Clinical Journal of the American Society of Nephrology
IS - 3
ER -