Increasing vimentin expression associated with differentiation of human and rat preadipocytes

K. Teichert-Kuliszewska, B. S. Hamilton, Dak Roncari, J. L. Kirkland, W. S. Gillon, M. Deitel, C. H. Hollenberg

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Alterations in the cytoskeletal apparatus constitute some of the earliest changes during assumption of an adipogenic phenotype. We examined three major cytoskeletal elements, β-actin, α-tubulin and vimentin, during adipogenesis in euploid cells from human and rat adipose tissue. As reported with 3T3 sub-lines, mRNA levels for β-actin and α-tubulin were decreased upon differentiation. However, in contrast to reports with 3T3 cells, levels of vimentin were increased during differentiation. Furthermore, immunological analyses confirmed that there was no decrease in vimentin protein levels during adipogenic development. As well as highlighting a difference between 3T3 cell lines and preadipocytes isolated from fat depots, these studies indicate that the pattern of cytoskeletal gene expression undergoes complex changes early during preadipocyte differentiation.

Original languageEnglish (US)
Pages (from-to)S108-S113
JournalInternational Journal of Obesity
Issue numberSUPPL. 3
StatePublished - 1996


  • Adipocytes
  • Differentiation
  • Obesity
  • Preadipocytes
  • Vimentin
  • α-tubulin
  • β-actin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics


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