Increased VLDL-TG fatty acid storage in skeletal muscle in men with type 2 diabeteS

Iben R. Andersen, Esben Søndergaard, Lars P. Sørensen, Birgitte Nellemann, Lars C. Gormsen, Michael D. Jensen, Søren Nielsen

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Context: Lipoprotein lipase (LPL) activity is considered the rate-limiting step of very-low-densitylipoprotein triglycerides (VLDL-TG) tissue storage, and has been suggested to relate to the development of obesity as well as insulin resistance and type 2 diabetes. Objective: The objective of the study was to assess the relationship between the quantitative storage of VLDL-TG fatty acids and LPL activity and other storage factors in muscle and adipose tissue. In addition, we examine whether such relations were influenced by type 2 diabetes. Design: We recruited 23 men (12 with type 2 diabetes, 11 nondiabetic) matched for age and body mass index. Postabsorptive VLDL-TG muscle and subcutaneous adipose tissue (abdominal and leg) quantitative storage was measured using tissue biopsies in combination with a primed-constant infusion of ex vivo triolein labeled [1-14C]VLDL-TG and a bolus infusion of ex vivo triolein labeled [9,10-3H]VLDL-TG. Biopsies were analyzed for LPL activity and cellular storage factors. Results: VLDL-TG storage rate was significantly greater in men with type 2 diabetes compared with nondiabetic men in muscle tissue (P = 0.02). We found no significant relationship between VLDL-TG storage rate and LPL activity or other storage factors in muscle or adipose tissue. However, LPL activity correlated with fractional VLDL-TG storage in abdominal fat (P = 0.04). Conclusions: Men with type 2 diabetes have increased VLDL-TG storage in muscle tissue, potentially contributing to increased intramyocellular triglyceride and ectopic lipid deposition.Neithermuscle nor adipose tissue storage rates were related to LPL activity. This argues against LPL as a rate-limiting step in the postabsorptive quantitative storage of VLDL-TG.

Original languageEnglish (US)
Pages (from-to)831-839
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Issue number3
StatePublished - Mar 1 2017

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


Dive into the research topics of 'Increased VLDL-TG fatty acid storage in skeletal muscle in men with type 2 diabeteS'. Together they form a unique fingerprint.

Cite this