In vivo tissue engineering of a trilayered leaflet-shaped tissue construct

Soumen Jana, Amir Lerman

Research output: Contribution to journalArticlepeer-review


Aim: We aimed to develop a leaflet-shaped trilayered tissue construct mimicking the morphology of native heart valve leaflets. Materials & methods: Electrospinning and in vivo tissue engineering methods were employed. Results: We developed leaflet-shaped microfibrous scaffolds, each with circumferentially, randomly and radially oriented three layers mimicking the trilayered, oriented structure of native leaflets. After 3 months in vivo tissue engineering with the scaffolds, the generated leaflet-shaped tissue constructs had a trilayered structure mimicking the orientations of native heart valve leaflets. Presence of collagen, glycosaminoglycans and elastin seen in native leaflets was observed in the engineered tissue constructs. Conclusion: Trilayered, oriented fibrous scaffolds brought the orientations of the infiltrated cells and their produced extracellular matrix proteins into the constructs.

Original languageEnglish (US)
Pages (from-to)1177-1192
Number of pages16
JournalRegenerative Medicine
Issue number1
StatePublished - Jan 2020


  • cardiac valve leaflet
  • in vivo
  • microfiber
  • tissue engineering
  • trilayered

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology


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