Abstract
Aim: We aimed to develop a leaflet-shaped trilayered tissue construct mimicking the morphology of native heart valve leaflets. Materials & methods: Electrospinning and in vivo tissue engineering methods were employed. Results: We developed leaflet-shaped microfibrous scaffolds, each with circumferentially, randomly and radially oriented three layers mimicking the trilayered, oriented structure of native leaflets. After 3 months in vivo tissue engineering with the scaffolds, the generated leaflet-shaped tissue constructs had a trilayered structure mimicking the orientations of native heart valve leaflets. Presence of collagen, glycosaminoglycans and elastin seen in native leaflets was observed in the engineered tissue constructs. Conclusion: Trilayered, oriented fibrous scaffolds brought the orientations of the infiltrated cells and their produced extracellular matrix proteins into the constructs.
Original language | English (US) |
---|---|
Pages (from-to) | 1177-1192 |
Number of pages | 16 |
Journal | Regenerative Medicine |
Volume | 15 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2020 |
Keywords
- cardiac valve leaflet
- in vivo
- microfiber
- tissue engineering
- trilayered
ASJC Scopus subject areas
- Biomedical Engineering
- Embryology