In situ hybridization in paracoccidioidomycosis

Thales De Brito, G. S. Sandhu, B. C. Kline, R. A. Aleff, M. P. Sandoval, R. T. Santos, A. A.H. Brandão, C. S. Lacaz

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

Original languageEnglish (US)
Pages (from-to)207-211
Number of pages5
JournalMedical mycology
Issue number3
StatePublished - Jun 1999


  • Human paracoccidioidomycosis
  • In situ hybridization
  • Oral biopsy
  • Paracoccidioides brasiliensis

ASJC Scopus subject areas

  • Infectious Diseases


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