Impact of atrial fibrillation on platelet gene expression

Waldemar E. Wysokinski, Alfonso Tafur, Naser Ammash, Samuel J. Asirvatham, Yanhong Wu, Izabella Gosk-Bierska, Diane E. Grill, Joshua P. Slusser, Jozef Mruk, Robert D. McBane

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Aims: Platelets retain cytoplasmic messenger RNA and are capable of protein biosynthesis. Several diseases are known to impact the platelet transcriptome but the effect of non-valvular atrial fibrillation (NVAF) on platelet RNA transcript is essentially unknown. The aim of this study was to evaluate the impact of NVAF on platelet RNA transcript by measuring platelet genes expression in consecutive NVAF patients before and 3-4 months after pulmonary vein isolation (PVI) and compared to normal sinus rhythm controls (NSR). Methods and Results: RNA from isolated platelets were reverse transcribed, assayed against 15 genes using real-time qPCR, and expressed as mean cycle threshold (ΔCt) using beta-2-microglobulin as endogenous control. Expression of all evaluated genes, except cathepsin A gene, was significantly lower (higher ΔCt) in 103 NVAF patients compared to 55 NSR controls. Insulin-like growth factor binding protein acid labile subunit gene (IGFALS) had expression more than 16 fold-lower (17.0±2.8 vs 12.5±3.8, P<.001), follow by genes encoding for prostacyclin receptor, and for von Willebrand factor which had fourfold lower expression compared to NSR controls. Gender, type of atrial fibrillation, heart failure, hypertension, prior stroke, diabetes mellitus, and atherosclerosis were associated with different gene expression. Following PVI, expression of four genes significantly increased, particularly IGFALS gene (increased 256-fold) and ADAMT gene increased 16-fold); expression of three genes significantly decreased, and expression of eight genes has not changed. Conclusions: Platelets are capable to respond to the circulatory environment of NVAF by altering transcript and changing prothrombotic status. This shows platelet potential for molecular “reprogramming” possibly induced by flow disturbances of NVAF.

Original languageEnglish (US)
Pages (from-to)615-621
Number of pages7
JournalEuropean Journal of Haematology
Issue number6
StatePublished - Jun 2017


  • gene expression
  • platelets
  • pulmonary vein isolation
  • thrombosis

ASJC Scopus subject areas

  • Hematology


Dive into the research topics of 'Impact of atrial fibrillation on platelet gene expression'. Together they form a unique fingerprint.

Cite this