Immunoblot Analysis and Band Depletion Assays

Scott H. Kaufmann, Phyllis A. Svingen

Research output: Chapter in Book/Report/Conference proceedingChapter


Western blotting has been widely utilized to detect various polypeptides or polypeptide epitopes (e.g., posttranslational modifications) within cells (reviewed in 1-4). If the signals in samples being analyzed are compared to a suitable standard curve and appropriate internal standards are utilized to confirm equivalent loading of various samples, Western blotting appears to be a suitable method of quantitating polypeptides as well.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages16
StatePublished - 1999

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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