Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer

Chowdhury Arif Jahangir; David B. Page; Glenn Broeckx; Claudia A. Gonzalez; Caoimbhe Burke; Clodagh Murphy; Jorge S. Reis-Filho; Amy Ly; Paul W. Harms; Rajarsi R. Gupta; Michael Vieth; Akira I. Hida; Mohamed Kahila; Zuzana Kos; Paul J. van Diest; Sara Verbandt; Jeppe Thagaard; Reena Khiroya; Khalid Abduljabbar; Gabriela Acosta Haab; Balazs Acs; Sylvia Adams; Jonas S. Almeida; Isabel Alvarado-Cabrero; Farid Azmoudeh-Ardalan; Sunil Badve; Nurkhairul Bariyah Baharun; Enrique R. Bellolio; Vydehi Bheemaraju; Kim R.M. Blenman; Luciana Botinelly Mendonça Fujimoto; Octavio Burgues; Alexandros Chardas; Maggie Chon U. Cheang; Francesco Ciompi; Lee A.D. Cooper; An Coosemans; Germán Corredor; Flavio Luis Dantas Portela; Frederik Deman; Sandra Demaria; Sarah N. Dudgeon; Mahmoud Elghazawy; Claudio Fernandez-Martín; Susan Fineberg; Stephen B. Fox; Jennifer M. Giltnane; Sacha Gnjatic; Paula I. Gonzalez-Ericsson; Anita Grigoriadis; Niels Halama; Matthew G. Hanna; Aparna Harbhajanka; Steven N. Hart; Johan Hartman; Stephen Hewitt; Hugo M. Horlings; Zaheed Husain; Sheeba Irshad; Emiel A.M. Janssen; Tatsuki R. Kataoka; Kosuke Kawaguchi; Andrey I. Khramtsov; Umay Kiraz; Pawan Kirtani; Liudmila L. Kodach; Konstanty Korski; Guray Akturk; Ely Scott; Anikó Kovács; Anne Vibeke Lænkholm; Corinna Lang-Schwarz; Denis Larsimont; Jochen K. Lennerz; Marvin Lerousseau; Xiaoxian Li; Anant Madabhushi; Sai K. Maley; Vidya Manur Narasimhamurthy; Douglas K. Marks; Elizabeth S. McDonald; Ravi Mehrotra; Stefan Michiels; Durga Kharidehal; Fayyaz ul Amir Afsar Minhas; Shachi Mittal; David A. Moore; Shamim Mushtaq; Hussain Nighat; Thomas Papathomas; Frederique Penault-Llorca; Rashindrie D. Perera; Christopher J. Pinard; Juan Carlos Pinto-Cardenas; Giancarlo Pruneri; Lajos Pusztai; Nasir Mahmood Rajpoot; Bernardo Leon Rapoport; Tilman T. Rau; Joana M. Ribeiro; David Rimm; Anne Vincent-Salomon; Joel Saltz; Shahin Sayed; Evangelos Hytopoulos; Sarah Mahon; Kalliopi P. Siziopikou; Christos Sotiriou; Albrecht Stenzinger; Maher A. Sughayer; Daniel Sur; Fraser Symmans; Sunao Tanaka; Timothy Taxter; Sabine Tejpar; Jonas Teuwen; E. Aubrey Thompson; Trine Tramm; William T. Tran; Jeroen van der Laak; Gregory E. Verghese; Giuseppe Viale; Noorul Wahab; Thomas Walter; Yannick Waumans; Hannah Y. Wen; Wentao Yang; Yinyin Yuan; John Bartlett; Sibylle Loibl; Carsten Denkert; Peter Savas; Sherene Loi; Elisabeth Specht Stovgaard; Roberto Salgado; William M. Gallagher; Arman Rahman

doi:10.1002/path.6238

Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer

Chowdhury Arif Jahangir, David B. Page, Glenn Broeckx, Claudia A. Gonzalez, Caoimbhe Burke, Clodagh Murphy, Jorge S. Reis-Filho, Amy Ly, Paul W. Harms, Rajarsi R. Gupta, Michael Vieth, Akira I. Hida, Mohamed Kahila, Zuzana Kos, Paul J. van Diest, Sara Verbandt, Jeppe Thagaard, Reena Khiroya, Khalid Abduljabbar, Gabriela Acosta HaabBalazs Acs, Sylvia Adams, Jonas S. Almeida, Isabel Alvarado-Cabrero, Farid Azmoudeh-Ardalan, Sunil Badve, Nurkhairul Bariyah Baharun, Enrique R. Bellolio, Vydehi Bheemaraju, Kim R.M. Blenman, Luciana Botinelly Mendonça Fujimoto, Octavio Burgues, Alexandros Chardas, Maggie Chon U. Cheang, Francesco Ciompi, Lee A.D. Cooper, An Coosemans, Germán Corredor, Flavio Luis Dantas Portela, Frederik Deman, Sandra Demaria, Sarah N. Dudgeon, Mahmoud Elghazawy, Claudio Fernandez-Martín, Susan Fineberg, Stephen B. Fox, Jennifer M. Giltnane, Sacha Gnjatic, Paula I. Gonzalez-Ericsson, Anita Grigoriadis, Niels Halama, Matthew G. Hanna, Aparna Harbhajanka, Steven N. Hart, Johan Hartman, Stephen Hewitt, Hugo M. Horlings, Zaheed Husain, Sheeba Irshad, Emiel A.M. Janssen, Tatsuki R. Kataoka, Kosuke Kawaguchi, Andrey I. Khramtsov, Umay Kiraz, Pawan Kirtani, Liudmila L. Kodach, Konstanty Korski, Guray Akturk, Ely Scott, Anikó Kovács, Anne Vibeke Lænkholm, Corinna Lang-Schwarz, Denis Larsimont, Jochen K. Lennerz, Marvin Lerousseau, Xiaoxian Li, Anant Madabhushi, Sai K. Maley, Vidya Manur Narasimhamurthy, Douglas K. Marks, Elizabeth S. McDonald, Ravi Mehrotra, Stefan Michiels, Durga Kharidehal, Fayyaz ul Amir Afsar Minhas, Shachi Mittal, David A. Moore, Shamim Mushtaq, Hussain Nighat, Thomas Papathomas, Frederique Penault-Llorca, Rashindrie D. Perera, Christopher J. Pinard, Juan Carlos Pinto-Cardenas, Giancarlo Pruneri, Lajos Pusztai, Nasir Mahmood Rajpoot, Bernardo Leon Rapoport, Tilman T. Rau, Joana M. Ribeiro, David Rimm, Anne Vincent-Salomon, Joel Saltz, Shahin Sayed, Evangelos Hytopoulos, Sarah Mahon, Kalliopi P. Siziopikou, Christos Sotiriou, Albrecht Stenzinger, Maher A. Sughayer, Daniel Sur, Fraser Symmans, Sunao Tanaka, Timothy Taxter, Sabine Tejpar, Jonas Teuwen, E. Aubrey Thompson, Trine Tramm, William T. Tran, Jeroen van der Laak, Gregory E. Verghese, Giuseppe Viale, Noorul Wahab, Thomas Walter, Yannick Waumans, Hannah Y. Wen, Wentao Yang, Yinyin Yuan, John Bartlett, Sibylle Loibl, Carsten Denkert, Peter Savas, Sherene Loi, Elisabeth Specht Stovgaard, Roberto Salgado, William M. Gallagher, Arman Rahman

Research output: Contribution to journal › Review article › peer-review

Abstract

Recent advances in the field of immuno-oncology have brought transformative changes in the management of cancer patients. The immune profile of tumours has been found to have key value in predicting disease prognosis and treatment response in various cancers. Multiplex immunohistochemistry and immunofluorescence have emerged as potent tools for the simultaneous detection of multiple protein biomarkers in a single tissue section, thereby expanding opportunities for molecular and immune profiling while preserving tissue samples. By establishing the phenotype of individual tumour cells when distributed within a mixed cell population, the identification of clinically relevant biomarkers with high-throughput multiplex immunophenotyping of tumour samples has great potential to guide appropriate treatment choices. Moreover, the emergence of novel multi-marker imaging approaches can now provide unprecedented insights into the tumour microenvironment, including the potential interplay between various cell types. However, there are significant challenges to widespread integration of these technologies in daily research and clinical practice. This review addresses the challenges and potential solutions within a structured framework of action from a regulatory and clinical trial perspective. New developments within the field of immunophenotyping using multiplexed tissue imaging platforms and associated digital pathology are also described, with a specific focus on translational implications across different subtypes of cancer.

Original language	English (US)
Pages (from-to)	271-288
Number of pages	18
Journal	Journal of Pathology
Volume	262
Issue number	3
DOIs	https://doi.org/10.1002/path.6238
State	Published - Mar 2024

Keywords

cancer prognosis
clinical integration
digital image analysis
multiplex imaging
multiplex immunofluorescence
multiplex immunohistochemistry
therapy response
tumour immune profiling
tumour infiltrating lymphocytes

ASJC Scopus subject areas

Pathology and Forensic Medicine

Access to Document

10.1002/path.6238

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Jahangir, C. A., Page, D. B., Broeckx, G., Gonzalez, C. A., Burke, C., Murphy, C., Reis-Filho, J. S., Ly, A., Harms, P. W., Gupta, R. R., Vieth, M., Hida, A. I., Kahila, M., Kos, Z., van Diest, P. J., Verbandt, S., Thagaard, J., Khiroya, R., Abduljabbar, K., ... Rahman, A. (2024). Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer. Journal of Pathology, 262(3), 271-288. https://doi.org/10.1002/path.6238

Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer. / Jahangir, Chowdhury Arif; Page, David B.; Broeckx, Glenn et al.
In: Journal of Pathology, Vol. 262, No. 3, 03.2024, p. 271-288.

Research output: Contribution to journal › Review article › peer-review

Jahangir, CA, Page, DB, Broeckx, G, Gonzalez, CA, Burke, C, Murphy, C, Reis-Filho, JS, Ly, A, Harms, PW, Gupta, RR, Vieth, M, Hida, AI, Kahila, M, Kos, Z, van Diest, PJ, Verbandt, S, Thagaard, J, Khiroya, R, Abduljabbar, K, Acosta Haab, G, Acs, B, Adams, S, Almeida, JS, Alvarado-Cabrero, I, Azmoudeh-Ardalan, F, Badve, S, Baharun, NB, Bellolio, ER, Bheemaraju, V, Blenman, KRM, Botinelly Mendonça Fujimoto, L, Burgues, O, Chardas, A, Cheang, MCU, Ciompi, F, Cooper, LAD, Coosemans, A, Corredor, G, Dantas Portela, FL, Deman, F, Demaria, S, Dudgeon, SN, Elghazawy, M, Fernandez-Martín, C, Fineberg, S, Fox, SB, Giltnane, JM, Gnjatic, S, Gonzalez-Ericsson, PI, Grigoriadis, A, Halama, N, Hanna, MG, Harbhajanka, A, Hart, SN, Hartman, J, Hewitt, S, Horlings, HM, Husain, Z, Irshad, S, Janssen, EAM, Kataoka, TR, Kawaguchi, K, Khramtsov, AI, Kiraz, U, Kirtani, P, Kodach, LL, Korski, K, Akturk, G, Scott, E, Kovács, A, Lænkholm, AV, Lang-Schwarz, C, Larsimont, D, Lennerz, JK, Lerousseau, M, Li, X, Madabhushi, A, Maley, SK, Manur Narasimhamurthy, V, Marks, DK, McDonald, ES, Mehrotra, R, Michiels, S, Kharidehal, D, Minhas, FUAA, Mittal, S, Moore, DA, Mushtaq, S, Nighat, H, Papathomas, T, Penault-Llorca, F, Perera, RD, Pinard, CJ, Pinto-Cardenas, JC, Pruneri, G, Pusztai, L, Rajpoot, NM, Rapoport, BL, Rau, TT, Ribeiro, JM, Rimm, D, Vincent-Salomon, A, Saltz, J, Sayed, S, Hytopoulos, E, Mahon, S, Siziopikou, KP, Sotiriou, C, Stenzinger, A, Sughayer, MA, Sur, D, Symmans, F, Tanaka, S, Taxter, T, Tejpar, S, Teuwen, J, Thompson, EA, Tramm, T, Tran, WT, van der Laak, J, Verghese, GE, Viale, G, Wahab, N, Walter, T, Waumans, Y, Wen, HY, Yang, W, Yuan, Y, Bartlett, J, Loibl, S, Denkert, C, Savas, P, Loi, S, Specht Stovgaard, E, Salgado, R, Gallagher, WM & Rahman, A 2024, 'Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer', Journal of Pathology, vol. 262, no. 3, pp. 271-288. https://doi.org/10.1002/path.6238

@article{9dc412b6cd774dcdab7825f26e5bd7e2,

title = "Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer",

abstract = "Recent advances in the field of immuno-oncology have brought transformative changes in the management of cancer patients. The immune profile of tumours has been found to have key value in predicting disease prognosis and treatment response in various cancers. Multiplex immunohistochemistry and immunofluorescence have emerged as potent tools for the simultaneous detection of multiple protein biomarkers in a single tissue section, thereby expanding opportunities for molecular and immune profiling while preserving tissue samples. By establishing the phenotype of individual tumour cells when distributed within a mixed cell population, the identification of clinically relevant biomarkers with high-throughput multiplex immunophenotyping of tumour samples has great potential to guide appropriate treatment choices. Moreover, the emergence of novel multi-marker imaging approaches can now provide unprecedented insights into the tumour microenvironment, including the potential interplay between various cell types. However, there are significant challenges to widespread integration of these technologies in daily research and clinical practice. This review addresses the challenges and potential solutions within a structured framework of action from a regulatory and clinical trial perspective. New developments within the field of immunophenotyping using multiplexed tissue imaging platforms and associated digital pathology are also described, with a specific focus on translational implications across different subtypes of cancer.",

keywords = "cancer prognosis, clinical integration, digital image analysis, multiplex imaging, multiplex immunofluorescence, multiplex immunohistochemistry, therapy response, tumour immune profiling, tumour infiltrating lymphocytes",

author = "Jahangir, {Chowdhury Arif} and Page, {David B.} and Glenn Broeckx and Gonzalez, {Claudia A.} and Caoimbhe Burke and Clodagh Murphy and Reis-Filho, {Jorge S.} and Amy Ly and Harms, {Paul W.} and Gupta, {Rajarsi R.} and Michael Vieth and Hida, {Akira I.} and Mohamed Kahila and Zuzana Kos and {van Diest}, {Paul J.} and Sara Verbandt and Jeppe Thagaard and Reena Khiroya and Khalid Abduljabbar and {Acosta Haab}, Gabriela and Balazs Acs and Sylvia Adams and Almeida, {Jonas S.} and Isabel Alvarado-Cabrero and Farid Azmoudeh-Ardalan and Sunil Badve and Baharun, {Nurkhairul Bariyah} and Bellolio, {Enrique R.} and Vydehi Bheemaraju and Blenman, {Kim R.M.} and {Botinelly Mendon{\c c}a Fujimoto}, Luciana and Octavio Burgues and Alexandros Chardas and Cheang, {Maggie Chon U.} and Francesco Ciompi and Cooper, {Lee A.D.} and An Coosemans and Germ{\'a}n Corredor and {Dantas Portela}, {Flavio Luis} and Frederik Deman and Sandra Demaria and Dudgeon, {Sarah N.} and Mahmoud Elghazawy and Claudio Fernandez-Mart{\'i}n and Susan Fineberg and Fox, {Stephen B.} and Giltnane, {Jennifer M.} and Sacha Gnjatic and Gonzalez-Ericsson, {Paula I.} and Anita Grigoriadis and Niels Halama and Hanna, {Matthew G.} and Aparna Harbhajanka and Hart, {Steven N.} and Johan Hartman and Stephen Hewitt and Horlings, {Hugo M.} and Zaheed Husain and Sheeba Irshad and Janssen, {Emiel A.M.} and Kataoka, {Tatsuki R.} and Kosuke Kawaguchi and Khramtsov, {Andrey I.} and Umay Kiraz and Pawan Kirtani and Kodach, {Liudmila L.} and Konstanty Korski and Guray Akturk and Ely Scott and Anik{\'o} Kov{\'a}cs and L{\ae}nkholm, {Anne Vibeke} and Corinna Lang-Schwarz and Denis Larsimont and Lennerz, {Jochen K.} and Marvin Lerousseau and Xiaoxian Li and Anant Madabhushi and Maley, {Sai K.} and {Manur Narasimhamurthy}, Vidya and Marks, {Douglas K.} and McDonald, {Elizabeth S.} and Ravi Mehrotra and Stefan Michiels and Durga Kharidehal and Minhas, {Fayyaz ul Amir Afsar} and Shachi Mittal and Moore, {David A.} and Shamim Mushtaq and Hussain Nighat and Thomas Papathomas and Frederique Penault-Llorca and Perera, {Rashindrie D.} and Pinard, {Christopher J.} and Pinto-Cardenas, {Juan Carlos} and Giancarlo Pruneri and Lajos Pusztai and Rajpoot, {Nasir Mahmood} and Rapoport, {Bernardo Leon} and Rau, {Tilman T.} and Ribeiro, {Joana M.} and David Rimm and Anne Vincent-Salomon and Joel Saltz and Shahin Sayed and Evangelos Hytopoulos and Sarah Mahon and Siziopikou, {Kalliopi P.} and Christos Sotiriou and Albrecht Stenzinger and Sughayer, {Maher A.} and Daniel Sur and Fraser Symmans and Sunao Tanaka and Timothy Taxter and Sabine Tejpar and Jonas Teuwen and Thompson, {E. Aubrey} and Trine Tramm and Tran, {William T.} and {van der Laak}, Jeroen and Verghese, {Gregory E.} and Giuseppe Viale and Noorul Wahab and Thomas Walter and Yannick Waumans and Wen, {Hannah Y.} and Wentao Yang and Yinyin Yuan and John Bartlett and Sibylle Loibl and Carsten Denkert and Peter Savas and Sherene Loi and {Specht Stovgaard}, Elisabeth and Roberto Salgado and Gallagher, {William M.} and Arman Rahman",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.",

year = "2024",

month = mar,

doi = "10.1002/path.6238",

language = "English (US)",

volume = "262",

pages = "271--288",

journal = "Journal of Pathology",

issn = "0022-3417",

publisher = "John Wiley and Sons Ltd",

number = "3",

}

TY - JOUR

T1 - Image-based multiplex immune profiling of cancer tissues

T2 - translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer

AU - Jahangir, Chowdhury Arif

AU - Page, David B.

AU - Broeckx, Glenn

AU - Gonzalez, Claudia A.

AU - Burke, Caoimbhe

AU - Murphy, Clodagh

AU - Reis-Filho, Jorge S.

AU - Ly, Amy

AU - Harms, Paul W.

AU - Gupta, Rajarsi R.

AU - Vieth, Michael

AU - Hida, Akira I.

AU - Kahila, Mohamed

AU - Kos, Zuzana

AU - van Diest, Paul J.

AU - Verbandt, Sara

AU - Thagaard, Jeppe

AU - Khiroya, Reena

AU - Abduljabbar, Khalid

AU - Acosta Haab, Gabriela

AU - Acs, Balazs

AU - Adams, Sylvia

AU - Almeida, Jonas S.

AU - Alvarado-Cabrero, Isabel

AU - Azmoudeh-Ardalan, Farid

AU - Badve, Sunil

AU - Baharun, Nurkhairul Bariyah

AU - Bellolio, Enrique R.

AU - Bheemaraju, Vydehi

AU - Blenman, Kim R.M.

AU - Botinelly Mendonça Fujimoto, Luciana

AU - Burgues, Octavio

AU - Chardas, Alexandros

AU - Cheang, Maggie Chon U.

AU - Ciompi, Francesco

AU - Cooper, Lee A.D.

AU - Coosemans, An

AU - Corredor, Germán

AU - Dantas Portela, Flavio Luis

AU - Deman, Frederik

AU - Demaria, Sandra

AU - Dudgeon, Sarah N.

AU - Elghazawy, Mahmoud

AU - Fernandez-Martín, Claudio

AU - Fineberg, Susan

AU - Fox, Stephen B.

AU - Giltnane, Jennifer M.

AU - Gnjatic, Sacha

AU - Gonzalez-Ericsson, Paula I.

AU - Grigoriadis, Anita

AU - Halama, Niels

AU - Hanna, Matthew G.

AU - Harbhajanka, Aparna

AU - Hart, Steven N.

AU - Hartman, Johan

AU - Hewitt, Stephen

AU - Horlings, Hugo M.

AU - Husain, Zaheed

AU - Irshad, Sheeba

AU - Janssen, Emiel A.M.

AU - Kataoka, Tatsuki R.

AU - Kawaguchi, Kosuke

AU - Khramtsov, Andrey I.

AU - Kiraz, Umay

AU - Kirtani, Pawan

AU - Kodach, Liudmila L.

AU - Korski, Konstanty

AU - Akturk, Guray

AU - Scott, Ely

AU - Kovács, Anikó

AU - Lænkholm, Anne Vibeke

AU - Lang-Schwarz, Corinna

AU - Larsimont, Denis

AU - Lennerz, Jochen K.

AU - Lerousseau, Marvin

AU - Li, Xiaoxian

AU - Madabhushi, Anant

AU - Maley, Sai K.

AU - Manur Narasimhamurthy, Vidya

AU - Marks, Douglas K.

AU - McDonald, Elizabeth S.

AU - Mehrotra, Ravi

AU - Michiels, Stefan

AU - Kharidehal, Durga

AU - Minhas, Fayyaz ul Amir Afsar

AU - Mittal, Shachi

AU - Moore, David A.

AU - Mushtaq, Shamim

AU - Nighat, Hussain

AU - Papathomas, Thomas

AU - Penault-Llorca, Frederique

AU - Perera, Rashindrie D.

AU - Pinard, Christopher J.

AU - Pinto-Cardenas, Juan Carlos

AU - Pruneri, Giancarlo

AU - Pusztai, Lajos

AU - Rajpoot, Nasir Mahmood

AU - Rapoport, Bernardo Leon

AU - Rau, Tilman T.

AU - Ribeiro, Joana M.

AU - Rimm, David

AU - Vincent-Salomon, Anne

AU - Saltz, Joel

AU - Sayed, Shahin

AU - Hytopoulos, Evangelos

AU - Mahon, Sarah

AU - Siziopikou, Kalliopi P.

AU - Sotiriou, Christos

AU - Stenzinger, Albrecht

AU - Sughayer, Maher A.

AU - Sur, Daniel

AU - Symmans, Fraser

AU - Tanaka, Sunao

AU - Taxter, Timothy

AU - Tejpar, Sabine

AU - Teuwen, Jonas

AU - Thompson, E. Aubrey

AU - Tramm, Trine

AU - Tran, William T.

AU - van der Laak, Jeroen

AU - Verghese, Gregory E.

AU - Viale, Giuseppe

AU - Wahab, Noorul

AU - Walter, Thomas

AU - Waumans, Yannick

AU - Wen, Hannah Y.

AU - Yang, Wentao

AU - Yuan, Yinyin

AU - Bartlett, John

AU - Loibl, Sibylle

AU - Denkert, Carsten

AU - Savas, Peter

AU - Loi, Sherene

AU - Specht Stovgaard, Elisabeth

AU - Salgado, Roberto

AU - Gallagher, William M.

AU - Rahman, Arman

PY - 2024/3

Y1 - 2024/3

N2 - Recent advances in the field of immuno-oncology have brought transformative changes in the management of cancer patients. The immune profile of tumours has been found to have key value in predicting disease prognosis and treatment response in various cancers. Multiplex immunohistochemistry and immunofluorescence have emerged as potent tools for the simultaneous detection of multiple protein biomarkers in a single tissue section, thereby expanding opportunities for molecular and immune profiling while preserving tissue samples. By establishing the phenotype of individual tumour cells when distributed within a mixed cell population, the identification of clinically relevant biomarkers with high-throughput multiplex immunophenotyping of tumour samples has great potential to guide appropriate treatment choices. Moreover, the emergence of novel multi-marker imaging approaches can now provide unprecedented insights into the tumour microenvironment, including the potential interplay between various cell types. However, there are significant challenges to widespread integration of these technologies in daily research and clinical practice. This review addresses the challenges and potential solutions within a structured framework of action from a regulatory and clinical trial perspective. New developments within the field of immunophenotyping using multiplexed tissue imaging platforms and associated digital pathology are also described, with a specific focus on translational implications across different subtypes of cancer.

AB - Recent advances in the field of immuno-oncology have brought transformative changes in the management of cancer patients. The immune profile of tumours has been found to have key value in predicting disease prognosis and treatment response in various cancers. Multiplex immunohistochemistry and immunofluorescence have emerged as potent tools for the simultaneous detection of multiple protein biomarkers in a single tissue section, thereby expanding opportunities for molecular and immune profiling while preserving tissue samples. By establishing the phenotype of individual tumour cells when distributed within a mixed cell population, the identification of clinically relevant biomarkers with high-throughput multiplex immunophenotyping of tumour samples has great potential to guide appropriate treatment choices. Moreover, the emergence of novel multi-marker imaging approaches can now provide unprecedented insights into the tumour microenvironment, including the potential interplay between various cell types. However, there are significant challenges to widespread integration of these technologies in daily research and clinical practice. This review addresses the challenges and potential solutions within a structured framework of action from a regulatory and clinical trial perspective. New developments within the field of immunophenotyping using multiplexed tissue imaging platforms and associated digital pathology are also described, with a specific focus on translational implications across different subtypes of cancer.

KW - cancer prognosis

KW - clinical integration

KW - digital image analysis

KW - multiplex imaging

KW - multiplex immunofluorescence

KW - multiplex immunohistochemistry

KW - therapy response

KW - tumour immune profiling

KW - tumour infiltrating lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=85182480446&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85182480446&partnerID=8YFLogxK

U2 - 10.1002/path.6238

DO - 10.1002/path.6238

M3 - Review article

C2 - 38230434

AN - SCOPUS:85182480446

SN - 0022-3417

VL - 262

SP - 271

EP - 288

JO - Journal of Pathology

JF - Journal of Pathology

IS - 3

ER -

Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer

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