TY - JOUR
T1 - Identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (Ckdu) in Sri Lanka
AU - Muthusinghe, Devinda S.
AU - Shimizu, Kenta
AU - Lokupathirage, Sithumini M.W.
AU - Wei, Zhouoxing
AU - Sarathkumara, Yomani D.
AU - Amanda Fonseka, G. R.
AU - Senarathne, Pavani
AU - Koizumi, Nobuo
AU - Kawakami, Tomonori
AU - Koizumi, Akio
AU - Wickramasinghe, Chaminda
AU - Ebihara, Hideki
AU - Matsuno, Keita
AU - Tsuda, Yoshimi
AU - Arikawa, Jiro
AU - Gamage, Chandika D.
AU - Yoshimatsu, Kumiko
N1 - Funding Information:
Funding: This research was funded by The Japanese Society for the Promotion of Science (JSPS) KAKENHI (19K10595, 16H06429, 16K21723, and 16H06431); U.S.–Japan Cooperative Medical Sciences Program Collaborative Awards, 2018 (AMED 18950142 and CRDF-Global 64067); Science and Technology Research Partnership for Sustainable Development (SATREPS 19jm0110019h0002); and Heiwa Nakajima Foundation. The National Science Foundation of Sri Lanka grant (RPHS/2016/CKDu/06) was received by Rohana Chandrajith (Study 03 PI—Chandika D. Gamage), Department of Geology, Faculty of Science, University of Peradeniya, Sri Lanka.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/10
Y1 - 2021/10
N2 - We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patients and rodents in Sri Lankan CKDu hotspots. We captured 116 small mammals from CKDu endemic regions in the Polonnaruwa District of Sri Lanka. Seven animals (five out of 11 Mus booduga and two out of 99 Rattus rattus) were PCR-positive for the hantavirus. A rat-borne sequence was grouped with a THAIV-like Anjozorobe virus. In contrast, Mus-borne sequences belonged to the THAIV lineage, suggesting a novel orthohantavirus species according to the phylogenetic analyses and whole-genome comparisons. Our genetic evidence indicates the presence of two THAIV-related viruses circulating in this CKDu endemic area, suggesting a basis for further investigations to identify the infectious virus in patients with CKDu and the CKDu induction mechanism of these viruses.
AB - We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patients and rodents in Sri Lankan CKDu hotspots. We captured 116 small mammals from CKDu endemic regions in the Polonnaruwa District of Sri Lanka. Seven animals (five out of 11 Mus booduga and two out of 99 Rattus rattus) were PCR-positive for the hantavirus. A rat-borne sequence was grouped with a THAIV-like Anjozorobe virus. In contrast, Mus-borne sequences belonged to the THAIV lineage, suggesting a novel orthohantavirus species according to the phylogenetic analyses and whole-genome comparisons. Our genetic evidence indicates the presence of two THAIV-related viruses circulating in this CKDu endemic area, suggesting a basis for further investigations to identify the infectious virus in patients with CKDu and the CKDu induction mechanism of these viruses.
KW - Anjozorobe hantavirus
KW - Hantavirus
KW - Mus booduga
KW - Thailand orthohantavirus
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U2 - 10.3390/v13101984
DO - 10.3390/v13101984
M3 - Article
C2 - 34696414
AN - SCOPUS:85116484261
SN - 1999-4915
VL - 13
JO - Viruses
JF - Viruses
IS - 10
M1 - 1984
ER -