Human Leukocyte Antigen Association Study Reveals DRB1∗04:02 Effects Additional to DRB1∗07:01 in Anti-LGI1 Encephalitis

Vicente Peris Sempere, Sergio Muñiz-Castrillo, Aditya Ambati, Sophie Binks, Anne Laurie Pinto, Veronique Rogemond, Sean J. Pittock, Divyanshu Dubey, Michael D. Geschwind, Jeffrey Marc Gelfand, Sonam Dilwali, Soon Tae Lee, Julian Knight, Katherine S. Elliott, Sarosh Irani, Jérôme Honnorat, Emmanuel Mignot

Research output: Contribution to journalArticlepeer-review


Background and Objective s : To study human leukocyte antigen (HLA) allele associations in anti-leucine-rich glioma-inactivated 1 (LGI1) encephalitis. Methods : A multiethnic cohort of 269 patients with anti-LGI1 encephalitis and 1,359 controls was included. Four-digit HLA sequencing and genome wide association single-nucleotide polymorphism typing imputation (0.99 concordance) were used for HLA typing. Significance of primary and secondary associations was tested using χ2, Fisher exact tests, or logistic regression with the control of population stratification covariates when applicable. Results : DRB1∗07:01 and DQA1∗02:01, 2 alleles in strong linkage disequilibrium, were associated with the disease (90% vs 24%, OR = 27.8, p < 10e-50) across ethnicity independent of variation at DRB3 and DQB1, 2 flanking HLA loci. DRB1∗07:01 homozygosity was associated with a doubling of risk (OR = 2.1, p = 0.010), suggesting causality. DRB1∗07:01 negative subjects were younger (p = 0.003) and more frequently female (p = 0.015). Three patients with malignant thymomas did not carry DRB1∗07:01, whereas patients with other tumors had high DRB1∗07:01 frequency, suggesting that the presence of tumors other than thymomas may be coincidental and not causal. In both DRB1∗07:01 heterozygous individuals and DRB1∗07:01 negative subjects, DRB1∗04:02 was associated with anti-LGI1 encephalitis, indicating an independent effect of this allele (OR = 6.85, p = 4.57 × 10-6 and OR = 8.93, p = 2.50 × 10-3, respectively). DRB1∗04:02 was also independently associated with younger age at onset (β = -6.68, p = 9.78 × 10-3). Major histocompatibility complex peptide-binding predictions using LGI1-derived peptides revealed divergent binding propensities for DRB1∗04:02 and DRB1∗07:01 alleles, suggesting independent pathogenic mechanisms. Discussion : In addition to the established primary DRB1∗07:01 association in anti-LGI1 encephalitis, we observe a secondary effect of DRB1∗04:02 with lower age at onset. Our study provides evidence for secondary effects within HLA locus that correlate with clinical phenotypes in anti-LGI1 encephalitis.

Original languageEnglish (US)
Article numbere1140
JournalNeurology: Neuroimmunology and NeuroInflammation
Issue number2
StatePublished - Mar 3 2022

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology


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