Human H1 Histone Gene Promoter CCAAT Box Binding Protein HiNF-B Is a Mosaic Factor

Andre J. Van Wijnen, Robert F. Massung, Janet L. Stein, Gary S. Stein

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Vertebrate histone gene promoters in many cases contain an upstream element, 5'dCCAAT, that has been implicated in modulating the efficiency of transcription of a broad spectrum of genes. We have previously isolated a nuclear factor (HiNF-B) that binds specifically to the CCAAT element of a cell cycle regulated human HI histone gene. This factor shows similarities with other CCAAT box binding proteins in that it recognizes the same sequence but shows a distinct chromatographic behavior. In the present study, we have employed the gel retardation assay to demonstrate that HiNF-B is a cell cycle independent DNA binding protein that is conserved in both human and mouse cells. Using a series of reconstitution experiments with partially purified HiNF-B fractions, we show that this factor requires association of at least two components for site-specific binding. The composite structure of HiNF-B suggests that binding of at least some CCAAT elements in vertebrates may require cooperative interaction of CCAAT box binding proteins with other factors.

Original languageEnglish (US)
Pages (from-to)6534-6541
Number of pages8
Issue number17
StatePublished - Aug 1 1988

ASJC Scopus subject areas

  • Biochemistry


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